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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Improving the X-ray resolution by reversible flash-cooling combined with concentration screening, as exemplified with PPase
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Improving the X-ray resolution by reversible flash-cooling combined with concentration screening, as exemplified with PPase

机译：提高x射线通过可逆的决议flash-cooling结合浓度筛选,与PPase例证

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A significant improvement in the X-ray resolution of crystals of Escherichia coli inorganic pyrophosphatase at cryotemperature was obtained as a result of studying the relationship between the crystal order and cryosolution component concentrations. To perform the experiments, the ability to reverse the flash-cooling process and to return a crystal to ambient temperature was used. In each cycle, the crystal was transferred from a cold nitrogen-gas stream to a cryosolution with modified concentrations of the components. The crystal was then flash-cooled again and the diffraction quality checked. Such a technique allows the screening of a wide concentration range rather quickly without using a large number of crystals and allows the determination of optimal cryosolution component concentrations. The resolution limit for crystals of pyrophosphatase increased by almost 0.7 A, from 1.8 to 1.15 A.
机译：显著提高x射线决议大肠杆菌无机晶体在cryotemperature焦磷酸酶由于学习的关系水晶秩序和cryosolution组件浓度。扭转flash-cooling过程和能力返回一个水晶环境温度使用。从冰冷的氮气流cryosolution修改后的浓度的组件。然后再次flash-cooled和水晶衍射质量检查。允许广泛的筛选浓度不使用大量范围很快晶体和允许的决心最优浓度cryosolution组件。晶体的分辨率极限焦磷酸酶几乎增加了0.7,1.8到1.15。

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