Crystallization and preliminary X-ray analysis of the matrix protein from Ebola virus

Andrea Dessen; Eric Forest; Viktor VolchkovOlga DolnikHans-Dieter KlenkWinfried Weissenhorn

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Crystallization and preliminary X-ray analysis of the matrix protein from Ebola virus

机译：结晶和初步的x射线分析埃博拉病毒的基质蛋白

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The matrix protein from Ebola virus is a membrane-associated molecule that plays a role in viral budding. Despite its functional similarity to other viral matrix proteins, it displays no sequence similarity and hence may have a distinct fold. X-ray diffraction quality crystals of the Ebola VP40 matrix protein were grown by the hanging-drop vapour-diffusion method. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 64.4, b = 91.1, c = 47.9 A, β = 96.3 deg. A data set to 1.9 A resolution has been collected using synchrotron radiation. The unit cell contains one molecule of molecular weight 35 kDa per asymmetric unit, with a corresponding volume solvent content of 35%.

机译：埃博拉病毒是一种基质蛋白膜相关分子起着作用病毒出芽。其他病毒基质蛋白,它显示没有序列相似性,因此可能会有截然不同的褶皱。埃博拉VP40基质蛋白都增长了悬滴vapour-diffusion方法。属于单斜晶体空间群C2,晶胞参数= 64.4,b = 91.1, c =47.9,β= 96.3度。数据设置为1.9使用同步加速器决议已经被收集辐射。分子量每不对称单位,35 kDa相应的体积溶剂含量的35%。

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《Acta crystallographica.Section D. Biological crystallography》 |2000年第6期|758-760|共3页
作者
Andrea Dessen; Eric Forest; Viktor VolchkovOlga DolnikHans-Dieter KlenkWinfried Weissenhorn;
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作者单位

European Molecular Biology Laboratory (EMBL), 6 Rue jules Horowitz, 38000 Grenoble, France;

Institut de Biologie Structurale Jean-Pierre Ebel, 41 Avenue des Martyrs, 38027 Grenoble, France;

Institute for Virology, Robert-Koch-Strasse 17, 35037 Marburg, GermanyEuropean Molecular Biology Laboratory (EMBL), 6 Rue Jules Horowitz, 38000 Grenoble, France;

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正文语种英语
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关键词
matrix proteins; X-ray analysis; synchrotron radiationEbolavirusCrystallizationunit cell;

机译：基质蛋白;x射线分析;同步加速器radiationEbolavirusCrystallizationunit细胞;

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Crystallization and preliminary X-ray analysis of the matrix protein from Ebola virus

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