Crystallization and preliminary X-ray analysis of high-alkaline pectate lyase

Masatake Akita; Atsuo Suzuki; Tohru KobayashiSusumu ItoTakashi Yamane

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Crystallization and preliminary X-ray analysis of high-alkaline pectate lyase

机译：结晶和初步的x射线分析高碱性果胶酸裂解酶

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Pel-15, a high-alkaline pectate lyase (pectate transeliminase; E. C. 4.2.2.2) from Bacillus sp. strain KSM-P15, has been crystallized using the hanging-drop vapour-diffusion method at 277 K. Two different crystal forms were obtained and preliminary X-ray diffraction data were collected from each crystal form at 100 K. Both forms belong to the orthorhombic space group P2_12_12_1 and contain one molecule per asymmetric unit. The unit-cell parameters of form I are a = 43.2 (2), b = 60.2 (2), c = 82.2 (2) A and those of form II are a = 42.9 (1), b = 43.4 (1), c = 105.9 (3) A. Diffraction data to a resolution of 1.5 A were collected from form II crystals using a synchrotron-radiation source.

机译：Pel-15,一个高碱性果胶酸裂解酶(果胶酸盐transeliminase;应变KSM-P15一直使用的结晶在277 K悬滴vapour-diffusion方法。两个不同的晶体形式得到和初步的x射线衍射数据收集从每个晶体形式在100 K。属于集团P2_12_12_1斜方晶系的空间和每个不对称单元包含一个分子。晶胞参数的形式我= 43.2 (2),b = 60.2 (2), c = 82.2(2)的形式= 42.9 (1), b = 43.4 (1), c = 105.9(3)。衍射数据分辨率为1.5从形式收集第二晶体使用电脑同步源。

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《Acta crystallographica.Section D. Biological crystallography》 |2000年第6期|749-750|共2页
作者
Masatake Akita; Atsuo Suzuki; Tohru KobayashiSusumu ItoTakashi Yamane;
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作者单位

Tochigi Research Laboratories, Kao Corporation, 2606 Akabane, Ichikai, Haga, Tochigi 321-3497, Japan;

Department of Biotechonogy and Biomaterial Chemistry, Graduate School of Engineering, Nagoya University, Chikusa-ku, Nagoya 464-8603, Japan;

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正文语种英语
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pectate transeliminase; X-ray analysis; crystalsbacillus speciesX-ray diffraction datacrystal outlineCrystallization;

机译：果胶酸盐transeliminase;x射线分析;crystalsbacillus speciesX-ray衍射datacrystal outlineCrystallization;

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Crystallization and preliminary X-ray analysis of high-alkaline pectate lyase

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