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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >The 1.30 A resolution structure of the Bacillus subtilis chorismate mutase catalytic homotrimer
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The 1.30 A resolution structure of the Bacillus subtilis chorismate mutase catalytic homotrimer

机译：1.30芽孢杆菌的一项决议结构细小chorismate变位酶催化homotrimer

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摘要

The crystal structure of the Bacillus subtilis chorismate mutase, an enzyme of the aromatic amino acids biosynthetic pathway, was determined to 1.30 A resolution. The structure of the homotrimer was determined by molecular replacement using orthorhombic crystals of space group P2_12_12_1 with unitcell parameters a = 52.2, b = 83.8, c = 86.0 A. The ABC trimer of the monoclinic crystal structure [Chook et al. (1994), J. Mol. Biol. 240, 476-500] was used as the starting model. The final coordinates are composed of three complete polypeptide chains of 127 amino-acid residues. In addition, there are nine sulfate ions, five glycerol molecules and 424 water molecules clearly visible in the structure. This structure was refined with aniosotropic temperature factors, has excellent geometry and a crystallographic R factor of 0.169 with an R_(free) of 0.236. The three active sites of the macromolecule are at the subunit interfaces, with residues from two subunits contributing to each site. This orthorhombic crystal form was grown using ammonium sulfate as the precipitant; glycerol was used as a cryoprotectant during data collection. A glycerol molecule and sulfate ion in each of the active sites was found mimicking a transition-state analog. In this structure, the C-terminal tails of the subunits of the trimer are hydrogen bonded to residues of the active site of neighboring trimers in the crystal and thus cross-link the molecules in the crystal lattice.
机译：枯草芽孢杆菌的晶体结构chorismate变位酶,酶的芳香氨基酸生物合成途径,确定1.30解决方案。homotrimer由分子决定更换使用斜方晶体的空间集团P2_12_12_1 unitcell参数=52.2, b = 83.8, c = 86.0。单斜晶体结构(鸡等。(1994), j·摩尔。杂志。240年,476 - 500)是用作初始模型。三个完整的多肽链组成127个氨基酸残基。9硫酸盐离子、5和甘油分子424年水分子中清晰可见结构。aniosotropic温度因素,具有良好的几何和晶体R因子为0.1690.236的R_(免费)。高分子的子单元从两个子单元接口,与残留导致每个站点。晶体生长使用硫酸铵作为形式沉淀剂;冷冻保护剂在数据收集。硫酸分子和离子的活跃网站被发现模拟过渡态模拟。三聚物的子单元的氢结合残留的邻近的活性部位三聚晶体,因此交联分子在晶格中。

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