Characterization, crystallization and preliminary X-ray diffraction analysis of acutohaemolysin, a haemolytic toxin from Agkistrodon acutus venom

Qing-qiu Huang; Xue-yong Zhu; Nan LiWen-han DengTian-bao ChenPing-fan RaoMai-kun TengLi-wen Niu

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Characterization, crystallization and preliminary X-ray diffraction analysis of acutohaemolysin, a haemolytic toxin from Agkistrodon acutus venom

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Characterization, crystallization and preliminary X-ray diffraction analysis of acutohaemolysin, a haemolytic toxin from Agkistrodon acutus venom

机译：特性、结晶和初步的acutohaemolysin的x射线衍射分析溶血性毒素从蕲蛇acutus毒液

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Acutohaemolysin, a phospholipase A_2(PLA_2) from the venom of the snake Agkistrodon acutus, has been isolated and purified to homogeneity by anion-exchange chromatography on a DEAE-Sepharose column followed by cation-exchange chromatography on a CM-Sepharose column. It is an alkaline protein with an isoelectric point of 10.5 and is comprised of a single polypeptide chain of 13 938 Da. Its N-terminal amino-acid sequence shows very high similarity to Lys49-type PLA_2 proteins from other snake venoms. Although its PLA_2 enzymatic activity is very low, acutohaemolysin has a strong indirect haemolytic activity and anticoagulant activity. Acutohaemolysin crystals with a diffraction limit of 1.60 A were obtained by the hanging-drop vapour-diffusion method. The crystals belong to the space group C2, with unit-cell parameters a = 45.30, b = 59.55, c = 46.13 A, β= 117.69°. The asymmetric unit contains one molecule.

机译：Acutohaemolysin,磷脂酶a₂(PLA_2)蛇蛇的毒液acutus,同质性的分离和纯化DEAE-Sepharose阴离子交换色谱法其次是阳离子交换色谱法列CM-Sepharose列。蛋白质的等电点10.5,938年13组成的单一多肽链哒。高相似性Lys49-type PLA_2蛋白质其他蛇毒液。活动很低,acutohaemolysin有间接溶血性活动和强劲抗凝活性。1.60的衍射极限由悬滴vapour-diffusion方法。水晶属于空间群C2,晶胞参数= 45.30,b = 59.55, c =46.13,β= 117.69°。一个分子。

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《Acta crystallographica.Section D. Biological crystallography》 |2000年第7期|907-911|共5页
作者
Qing-qiu Huang; Xue-yong Zhu; Nan LiWen-han DengTian-bao ChenPing-fan RaoMai-kun TengLi-wen Niu;
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作者单位

Laboratory of Structural Biology, University of Science and Technology of China, CAS, 96 Jinzhai Road, Hefei, Anhui 230026, People's Republic of China;

Institute of Biotechnology, Fuzhou University, 523 Industry Road, Fuzhou, Fujian 350002, People's Republic of China;

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Isoelectric Point; Snake Venoms; VenomsAgkistrodon acutusX-ray diffraction analysisanion exchange chromatographyCation exchange chromatography;

机译：等电点;蛇毒液;VenomsAgkistrodonacutusX-ray衍射analysisanion交换chromatographyCation交换色谱法;

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Characterization, crystallization and preliminary X-ray diffraction analysis of acutohaemolysin, a haemolytic toxin from Agkistrodon acutus venom

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