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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Enhancement in the perfection of orthorhombic Iysozyme crystals grown in a high magnetic field (10 T)
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Enhancement in the perfection of orthorhombic Iysozyme crystals grown in a high magnetic field (10 T)

机译：增强斜方晶系的完美的Iysozyme晶体生长在高磁场(10 T)

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Orthorhombic crystals of hen egg-white (HEW) lysozyme were grown in a homogeneous and static magnetic field of 10 T. All crystals grown at 10 T were oriented such that their crystallographic c axes were parallel to the magnetic field direction and showed a narrower average full-width at half-maximum (FWHM) of the rocking curve than those grown at 0 T. Rocking-width measurements were made at the BL-10A station at the Photon Factory, Tsukuba, Japan, using a high-resolution vertical-type four-circle diffractometer. Crystal perfection was evaluated using the FWHM of the rocking curve; the effects of the magnetic field on the quality of the crystals were examined by comparison of the FWHM of seven crystals grown at 10 and 0 T. The FWHMs of the reflections along the a, b and c axes decreased by 23.5, 35.3 and 27.8%, respectively, and those of other general reflections decreased by 17.4-42.2% in the crystals grown at high magnetic field. These results clearly showed that a magnetic field of 10 T improved the crystal perfection of the orthorhombic lysozyme crystals. As a result, the maximum resolution of X-ray diffraction increased from 1.3 A at 0 T to 1.13 A at 10 T. The magnetic field also affected the dimensions of the unit cell, increments being 0.2% for the a and c axes and 0.1% for the b axis, respectively. These facts suggest that the application of a high magnetic field during crystallization might result in remarkable enhancements in the diffraction power of protein crystals having magnetic anisotropy.
机译：斜方晶系的母鸡的晶体蛋白(坚持)溶菌酶是在均匀和静态长大的磁场10 t .所有晶体生长T是面向这样的晶体c轴平行于磁场方向和显示一个狭义的平均水平宽屏在摇摆的半峰(应用)比增长0 t . Rocking-width曲线在BL-10A站进行了测量光子工厂,筑波,日本,使用高分辨率立式四个圆圈衍射仪。使用摇摆曲线的应用;磁场的质量晶体被应用的比较研究七晶体生长在10 0 t .应用的倒影在a、b和c轴分别下降了23.5、35.3和27.8%,和其他一般反射降低在晶体生长在高了17.4 -42.2%磁场。10 T的磁场改善晶体完美的斜方晶系的溶菌酶晶体。因此,x射线的最大分辨率衍射从0 T 1.3增加到1.1310 t磁场也影响了尺寸的单位细胞,增量0.2%的b和c轴和0.1%分别轴。高磁场时的应用结晶可能导致显著增强衍射能力的蛋白质晶体磁各向异性。

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