Crystallization and preliminary X-ray crystllographci studies on maltosyltransferase from Thermotoga maritima

Jacky Burke; Anna Roujeinikova; Patrick J. BakerSvetlana SedelnikovaCarsten RaaschWolfgang LieblDavid W. Rice

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Crystallization and preliminary X-ray crystllographci studies on maltosyltransferase from Thermotoga maritima

机译：结晶和初步的x射线crystllographci maltosyltransferase研究从Thermotoga maritima

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Thermotoga maritima maltosyltransferase (MTase) is a 73.7 kDa molecular weight amylolytic enzyme which catalyzes the transfer of maltosyl units from maltodextrins or starch to suitable acceptors. Crystals of recombinant MTase have been obtained by the hangingdrop vapour-diffusion method using ammonium phosphate as a precipitating agent. The crystals belong to space group P4_122 or its enantiomorph P4_322, with unit-cell parameters a = b = 148.7, c = 106.7 A. The asymmetric unit appears to contain one subunit, corresponding to a very low packing density of 4.0 A~3 Da~(-1). The crystals diffract X-rays to at least 2.4 A resolution on a synchrotronradiation source.

机译：Thermotoga maritima maltosyltransferase (MTase)73.7 kDa分子量淀粉分解的酶催化转移maltosyl单位从麦芽糊精或合适的淀粉受体。获得由hangingdrop vapour-diffusion方法用磷酸铵作为沉淀剂。集团P4_122或其对映体P4_322,晶胞参数a = b = 148.7, c = 106.7。不对称单元包含一个子单元,对应于一个非常低的包装密度4.0 ~ 3哒~(1)。x射线至少2.4一个决议synchrotronradiation来源。

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《Acta crystallographica.Section D. Biological crystallography》 |2000年第8期|1049-1050|共2页
作者
Jacky Burke; Anna Roujeinikova; Patrick J. BakerSvetlana SedelnikovaCarsten RaaschWolfgang LieblDavid W. Rice;
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作者单位

Institut fur Mikrobilogie und Genetik, Georg-August-Universitat, Gottingen, Germany;

Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield S10 2TN, England;

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Bulk density; ammonium phosphates; Thermotoga maritimamaltodextrinmaltosyltransferasecrystalsX-raysCrystallization;

机译：体积密度,铵磷酸盐;Thermotoga mariti;

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Crystallization and preliminary X-ray crystllographci studies on maltosyltransferase from Thermotoga maritima

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