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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and X-ray diffraction analysis of peroxisomal Δ~3-Δ~2-enoyl-CoA isomerase from Saccharomyces cerevisiae
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Crystallization and X-ray diffraction analysis of peroxisomal Δ~3-Δ~2-enoyl-CoA isomerase from Saccharomyces cerevisiae

机译：结晶和x射线衍射分析过氧化物酶病Δ~ 3 -Δ~ 2-enoyl-CoA异构酶酿酒酵母

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摘要

The purification, crystallization and X-ray diffraction analysis of Saccharomyces cerevisiae Δ~3-Δ~2-enoyl-CoA isomerase is described. Δ~3-Δ~2-Enoyl-CoA isomerase is a member of the hydratase/isomerase protein family and is an auxiliary enzyme required for the β-oxidation of unsaturated fatty acids. It is a hexameric enzyme consisting of six identical 32 kDa subunits of 280 residues each. In crystallization trials three crystal forms were obtained, with tetragonal and hexagonal lattices. A 2.5 A data set was collected from the unliganded hexagonal crystals with an R_(merge) of 6.6%. The crystal, with unit-cell parameters a = 116.0, b = 116.0, c = 122.9 A, is likely to have P6_322 symmetry.
机译：净化,结晶和x射线酿酒酵母的衍射分析Δ~ 3 -Δ~ 2-enoyl-CoA异构酶。Δ~ 3 -Δ~ 2-Enoyl-CoA异构酶的一员水合酶/异构酶蛋白家族,是一个所需的辅助酶β氧化不饱和脂肪酸。六个相同的32 kDa的子单元组成280残留。三个晶体形式,正方和六角晶格。设置收集unliganded六角晶体的R_(合并)的6.6%。晶胞参数= 116.0,= 116.0 b, c= 122.9,可能是P6_322对称。

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