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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >A general method for co-crystallization of concanavalin A with carbohydrates.
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A general method for co-crystallization of concanavalin A with carbohydrates.

机译：co-crystallization的一般方法伴刀豆球蛋白A和碳水化合物。

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摘要

A small grid of conditions has been developed for co-crystallization of the plant lectin concanavalin A (conA) and polysaccharides. Crystals have been obtained of complexes of conA with alpha1-2 mannobiose, 1-methyl alpha1-2 mannobiose, fructose, a trisaccharide and a pentasaccharide. The crystals diffract to resolutions of 1. 75-2.7 A using a copper rotating-anode source. The crystals are grown in the presence of polyethylene glycol 6K [10-20%(w/v)] at around pH 6.0. Optimization for each particular carbohydrate requires small adjustments in the conditions; however, all complexes give some crystalline precipitate in this limited grid. The alpha1-2 mannobiose complex crystals diffract to 1.75 A with space group I222 and cell dimensions a = 91.7, b = 86.8, c = 66.6 A. One monomer is present in the asymmetric unit. The 1-methyl alpha1-2 mannobioside complex crystallizes in space group P212121, cell dimensions a = 119. 7, b = 119.7, c = 68.9 A and diffract to 2.75 A. One tetramer is present in the asymmetric unit. Two crystal forms of the conA-fructose complex have been obtained. The first has space group P212121, cell dimensions a = 121.7, b = 119.9, c = 67.3 A with a tetramer in the asymmetric unit and diffracts to 2.6 A. The second crystallizes in space group C2221, cell dimensions a = 103.3, b = 117.9, c = 254.3 A with two dimers in the asymmetric unit and diffracts to 2.42 A. Structures and crystallization of the trisaccharide-conA and pentasaccharide-conA complexes have already been reported. In all complexes, the protein is found as a tetramer, although varying combinations of non-crystallographic and crystallographic symmetry are involved in generating the tetramer. The precise packing of the tetramer varies from crystal to crystal and it is likely that this variability facilitates crystallization.
机译：一个小网格的条件了co-crystallization植物凝集素伴刀豆球蛋白(conA)和多糖。取得了晶体conA的复合物与alpha1-2 mannobiose, 1号alpha1-2mannobiose,三糖和果糖pentasaccharide。分辨率为1。旋转阳极的来源。聚乙二醇的存在6 k[10 - 20% (w / v)]在pH值为6.0左右。每个特定碳水化合物需要小调整的条件;复合物给一些晶形沉淀这个有限的网格。1.75复杂晶体衍射与空间I222组和b细胞尺寸= 91.7,=86.8, c = 66.6。不对称单元。在空间群mannobioside复杂的结晶P212121,细胞尺寸= 119。2.75 = 68.9 A和衍射。出现在不对称单位。conA-fructose复杂。第一空间群P212121,细胞维a = 121.7, = 119.9 b, c = 67.3四聚物的不对称单元和期刊2.6。C2221,细胞尺寸= 103.3,b = 117.9, c =254.3有两个二聚体的不对称单元和2.42期刊。trisaccharide-conA和结晶pentasaccharide-conA复合体已经报道。四聚物,虽然不同的组合non-crystallographic和晶体对称参与生成四聚物。四聚物的精确的包装不同水晶,水晶,很可能这个变化促进了结晶。

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