Expression, purification, crystallization and preliminary X-ray diffraction analysis of uracil phosphoribosyltransferase of Toxoplasma gondii.

Barchue J; Narayana SV; Moore JKDeLucas LJChattopadhyay DSymersky J

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Expression, purification, crystallization and preliminary X-ray diffraction analysis of uracil phosphoribosyltransferase of Toxoplasma gondii.

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Expression, purification, crystallization and preliminary X-ray diffraction analysis of uracil phosphoribosyltransferase of Toxoplasma gondii.

机译：表达,纯化,结晶尿嘧啶的初步x射线衍射分析phosphoribosyltransferase刚地弓形虫。

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Recombinant uracil phosphoribosyltransferase (UPRT) enzyme of Toxoplasma gondii was expressed in Escherichia coli and purified from the cell-free extract by a combination of chromatographic steps. The recombinant protein was enzymatically active when tested in an in vitro UPRT assay. The purified protein was crystallized using the hanging-drop vapor-diffusion technique with ammonium phosphate as precipitant. The crystallized protein also exhibited UPRT activity. Crystals diffract to 2.4 A resolution and belong to space group P3121 or P3221 with unit-cell dimensions a = b = 119.9, c = 70.8 A and two molecules per asymmetric unit.

机译：尿嘧啶phosphoribosyltransferase重组刚地弓形虫(UPRT)酶是表达在大肠杆菌和净化无细胞提取物的组合色谱步骤。时保持酶的活性测试的体外UPRT化验。使用悬滴结晶扩散与磷铵技术作为沉淀剂。UPRT展出活动。一项决议,属于组P3121或空间P3221单胞尺寸= b = 119.9, c= 70.8和两个分子不对称单元。

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《Acta crystallographica.Section D. Biological crystallography》 |1999年第1期|347-349|共3页
作者
Barchue J; Narayana SV; Moore JKDeLucas LJChattopadhyay DSymersky J;
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Center for Macromolecular Crystallography, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, AL 35294, USA.;

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正文语种英语
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ammonium phosphates; Crystallography; AnimalsToxoplasma gondiiuracil phosphoribosyltransferaseX-ray diffraction analysisX-raysCrystallization;

机译：铵磷酸盐;结晶学;AnimalsToxoplasmagondiiuracil phosphoribosyltransferaseX-ray衍射analysisX-raysCrystallization;

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Expression, purification, crystallization and preliminary X-ray diffraction analysis of uracil phosphoribosyltransferase of Toxoplasma gondii.

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