Medium temperature, 310 K, provides single crystals of orotate phosphoribosyltransferase from Thermus thermophilus.

Hamana H; Shinozawa T; Tanaka NMoriyama H

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Medium temperature, 310 K, provides single crystals of orotate phosphoribosyltransferase from Thermus thermophilus.

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Medium temperature, 310 K, provides single crystals of orotate phosphoribosyltransferase from Thermus thermophilus.

机译：中温度310 K,提供单身晶体的orotate phosphoribosyltransferase从栖热菌属酸奶。

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摘要

Crystallization of orotate phosphoribosyltransferase from a thermophilic organism, Thermus thermophilus, was achieved using the hanging-drop vapour-diffusion method coupled with a macroseeding starter. Small needle-like microcrystals were grown in a fresh protein solution in the presence of 2-methyl-2,4-pentanediol at 298 K or below. Although these normal temperature conditions caused stacking crystallization, an increase of temperature to 310 K permitted crystal growth. This was because of increased enzyme solubility at the higher temperature. The crystal was found to belong to the monoclinic space group P21with unit-cell parameters a = 44.4, b = 59.6, c = 67.8 A and beta = 98.3 degrees.

机译：结晶的orotatephosphoribosyltransferase从嗜热生物体,栖热菌属酸奶,是实现使用悬滴vapour-diffusion方法再加上macroseeding起动器。针状的微晶核生长在一个新鲜蛋白质溶液的存在2-methyl-2 4-pentanediol在298 K以下。虽然这些正常的温度条件造成堆积结晶,增加温度310 K允许晶体生长。这是由于酶溶解度增加在更高的温度。属于单斜晶体的空间群P21with晶胞参数= 44.4,= 59.6 b, c = 67.8和β= 98.3度。

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来源
《Acta crystallographica.Section D. Biological crystallography》 |1999年第1期|345-346|共2页
作者
Hamana H; Shinozawa T; Tanaka NMoriyama H;
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作者单位

Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, Kiryu Gunma 376, Japan.;

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正文语种英语
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关键词
single crystals; microcrystals; Thermus thermophilusCrystallographyOrotate PhosphoribosyltransferaseTemperatureVital SignsX-raysTEMPERATURE RANGE 0273-0400 KCrystallization;

机译：单一晶体;微晶核,栖热菌属thermophilusCrystallographyOrotatePhosphoribosyltransferaseTemperatureVitalSignsX-raysTEMPERATURE范围0273 - 0400KCrystallization;

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Medium temperature, 310 K, provides single crystals of orotate phosphoribosyltransferase from Thermus thermophilus.

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