Expression, purification and preliminary X-ray analysis of a fibrillarin homolog from Methanococcus jannaschii, a hyperthermophile.

Wang H; Kim R; Kim SHYokota H

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Expression, purification and preliminary X-ray analysis of a fibrillarin homolog from Methanococcus jannaschii, a hyperthermophile.

机译：表达、纯化和初步的x射线分析fibrillarin同族体产甲烷球菌属jannaschii超嗜热菌。

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Fibrillarin plays a central role in ribosome biogenesis as a ribosomal RNA-processing protein. A Methanococcus jannaschii homolog of fibrillarin has been overexpressed, purified and crystallized. Crystals belong to the C2 space group with unit-cell parameters a = 121.4, b = 43.2, c = 55.3 A, beta = 96.9 degrees. Under flash-frozen conditions and using synchrotron radiation, the crystals diffract to 1.8 A resolution. For structural determination, a selenomethionine derivative of the protein has also been crystallized.

机译：Fibrillarin核糖体中起着重要的作用生物起源的核糖体rna加工蛋白质。一个产甲烷球菌属jannaschii fibrillarin同族体过表达,纯化,结晶。b组与晶胞参数= 121.4,=43.2, c = 55.3,β= 96.9度。瞬间冷冻条件和使用同步加速器辐射,1.8的晶体衍射决议。硒代蛋氨酸导数的蛋白质结晶。

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《Acta crystallographica.Section D. Biological crystallography》 |1999年第1期|338-340|共3页
作者
Wang H; Kim R; Kim SHYokota H;
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Department of Chemistry and Physical Biosciences Division, Lawrence Berkeley National Laboratory, University of California Berkeley, Berkeley, CA 94720-5230, USA.;

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正文语种英语
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X-ray analysis; Crystallography; Methanocaldococcus jannaschiiBase SequenceAquifex aeolicusX-raysfibrillarinCrystallization;

机译：x射线分析、晶体学、MethanocaldococcusjannaschiiBase SequenceAquifexaeolicusX-raysfibrillarinCrystallization;

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Expression, purification and preliminary X-ray analysis of a fibrillarin homolog from Methanococcus jannaschii, a hyperthermophile.

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