Preliminary characterization of crystals of an in vitro evolved cyanocobalamin (vitamin B12) binding RNA.

Sussman D; Reilly K; Wilson CGreensides D

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Preliminary characterization of crystals of an in vitro evolved cyanocobalamin (vitamin B12) binding RNA.

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Preliminary characterization of crystals of an in vitro evolved cyanocobalamin (vitamin B12) binding RNA.

机译：的初步表征晶体的一个体外进化维生素B12(维生素B12)RNA印象深刻。

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摘要

A 35-nucleotide pseudoknot that binds vitamin B12 been isolated using systematic evolution of ligands by exponential enrichment (SELEX). Affinity chromatography was used to purify functional, properly folded molecules and the hanging-drop vapor-diffusion method was used to crystallize this aptamer. Two crystal forms have been obtained and the preliminary crystallographic characterization is reported here. Both crystal forms (space groups I222 or I212121 and C2221) diffract to 2.9 A and should prove sufficient for structure determination.

机译：一个结合维生素B12 35-nucleotide赝结使用系统的演化被隔离由指数富集配体(SELEX)。亲和色谱法被用来净化功能,正确折叠的分子悬滴扩散法这个适体结晶。获得初步晶体特性报告在这里。2.9 I212121和C2221)衍射和应证明满足结构的决心。

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《Acta crystallographica.Section D. Biological crystallography》 |1999年第1期|326-328|共3页
作者
Sussman D; Reilly K; Wilson CGreensides D;
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作者单位

Department of Biology and Center for the Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz 95064, USA.;

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正文语种英语
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关键词
molecular evolution; Vitamin B 12; Directed Molecular Evolutiondirected evolutionCrystallographycrystalsBase SequenceCrystallizationCyanocobalaminNucleic Acid ConformationBinding SitesMolecular Sequence DataSELEX Aptamer TechniqueRNA metabolismX-rays;

机译：分子进化,维生素B 12;指导分子EvolutiondirectedevolutionCrystallographycrystalsBaseSequenceCrystallizationCyanocobalaminNucleic酸ConformationBinding SitesMolecular序列DataSELEX适配子TechniqueRNA metabolismX-rays;

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机译：化学和物理增强技术对Cyanocobalamin（Vitamin B12）透皮递送的影响

Preliminary characterization of crystals of an in vitro evolved cyanocobalamin (vitamin B12) binding RNA.

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