Crystallization and preliminary X-ray analysis of human RCC1, the regulator of chromosome condensation.

Renault L; Wittinghofer A; Roth MVetter IRNassar N

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Crystallization and preliminary X-ray analysis of human RCC1, the regulator of chromosome condensation.

机译：结晶和初步的x射线分析人类RCC1染色体的监管机构凝结。

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RCC1, the regulator of chromosome condensation, is the guanine nucleotide-exchange factor (GEF) of the GTP-binding protein Ran. Its GEF activity on Ran makes it a key element in nucleo-cytoplasmic transport and cell-cycle regulation. Crystals of human RCC1 suitable for X-ray analysis have been obtained using the seeding technique in hanging drops with sodium citrate as a precipitant. The crystals diffract to 1.7 A at 100 K and belong to the space group P1, with unit-cell parameters a = 49.5, b = 84.3, c = 84.9 A, alpha = 113.0, beta = 103.9,gamma = 103.3 degrees. The Matthews parameter (Vm) and the self-rotation function are consistent with three molecules in the unit cell, which is confirmed by the averaged single isomorphous replacement (SIR) electron-density map.

机译：RCC1,染色体缩合的调节器,是鸟嘌呤nucleotide-exchange因素(GEF)GTP-binding蛋白。跑使其质核的一个关键因素运输和细胞循环监管。人类RCC1适合x射线分析获得使用播种技术在挂滴与柠檬酸钠作为沉淀剂。晶体衍射在100 K,属于1.7空间群P1,晶胞参数=49.5, b = 84.3, c = 84.9,α= 113.0,β=103.9,γ= 103.3度。参数(Vm)和固有转动功能符合三个单元细胞中的分子,由平均单确认是哪一个同形替换(先生)电子密度地图。

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来源
《Acta crystallographica.Section D. Biological crystallography》 |1999年第1期|272-275|共4页
作者
Renault L; Wittinghofer A; Roth MVetter IRNassar N;
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Max-Planck-Institut fur molekulare Physiologie, Rheinlanddamm 201, 44139 Dortmund, Germany.;

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正文语种英语
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Human; Nucleo cytoplasmic Transport; X-ray analysisRCC1 geneCrystallographyRegulatorsChromosome CondensationX-raysCrystallizationunit cell;

机译：人类;质核运输;x射线analysisRCC1geneCrystallographyRegulatorsChromosomeCondensationX-raysCrystallizationunit细胞;

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Crystallization and preliminary X-ray analysis of human RCC1, the regulator of chromosome condensation.

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