Crystallization and preliminary X-ray diffraction studies of Streptococcus pyogenes plasmid pSM19035-encoded omega transcriptional repressor.

Murayama K; Alings C; Lopez GOrth PAlonso JCSaenger Wde la Hoz AB

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Crystallization and preliminary X-ray diffraction studies of Streptococcus pyogenes plasmid pSM19035-encoded omega transcriptional repressor.

机译：结晶和初步的x射线衍射酿脓链球菌质粒的研究pSM19035-encodedω转录抑制因子。

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The transcriptional repressor, omega protein, from the Streptococcus pyogenes broad-host-range plasmid pSM19035 was crystallized at pH 7. 5 and 8.5 by the vapour-diffusion method using PEG 4000 as precipitant. Two crystal forms were obtained; the first belongs to the tetragonal space group P4(1)2(1)2 or P4(3)2(1)2 and the second to the hexagonal space group P6(1) or P6(5). The crystals are most likely to contain one omega protein in the asymmetric unit, with V(m) values of 3.2 and 3.5 A(3) Da(-1), respectively. The crystals diffract X-rays to 2.4 and 2.9 A resolution for the tetragonal and hexagonal systems, -respectively.

机译：转录抑制因子,ω蛋白质,酿脓链球菌的broad-host-range质粒pSM19035结晶在pH值7。8.5 4000 vapour-diffusion方法使用挂钩作为沉淀剂。第一个属于正方空间群P4(1) 2(1) 2或P4(3) 2(1)和第二个六角空间群P6(1)或P6(5)。水晶是最有可能包含一个ω蛋白质在不对称单位,与V (m)值3.2和3.5 (3)Da(1),分别。x射线晶体衍射,2.4和2.9正方的决议和六角形系统,分别。

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《Acta crystallographica.Section D. Biological crystallography》 |1999年第12期|2041-2042|共2页
作者
Murayama K; Alings C; Lopez GOrth PAlonso JCSaenger Wde la Hoz AB;
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Institut fur Kristallographie, Freie Universitat Berlin, Takustrasse 6, D-14195 Berlin, Germany.;

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Plasmids; Crystallography; X-ray diffractioncrystalsspace groupsTranscription Repressor/CorepressorX-raysStreptococcus pyogenesCrystallization;

机译：质粒、晶体学、x射线diffractioncrystalsspace groupsTranscription阻遏物/ CorepressorX-raysStreptococcuspyogenesCrystallization;

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Crystallization and preliminary X-ray diffraction studies of Streptococcus pyogenes plasmid pSM19035-encoded omega transcriptional repressor.

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