Crystallization and preliminary X-ray diffraction studies of a DNA excision repair enzyme, UvrB, from Thermus thermophilus HB8.

Shibata A; Sugahara M; Masui RKato RKuramitsu SFukuyama KNakagawa N

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray diffraction studies of a DNA excision repair enzyme, UvrB, from Thermus thermophilus HB8.

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Crystallization and preliminary X-ray diffraction studies of a DNA excision repair enzyme, UvrB, from Thermus thermophilus HB8.

机译：结晶和初步的x射线衍射UvrB DNA切除修复酶的研究,从栖热菌属酸奶HB8。

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A DNA excision repair enzyme, UvrB, from Thermus thermophilus HB8 was crystallized by the vapor-diffusion method using lithium sulfate as the precipitant and beta-octylglucoside as an additive. The crystals belong to the trigonal space group P3121 or P3221, with unit-cell dimensions of a = b = 136.0 and c = 108.1 A. The crystal is most likely to contain one UvrB protein in an asymmetric unit with the Vm value of 3.8 A3 Da-1. The crystals diffracted X-rays beyond 2.9 A resolution. Although the crystals were sensitive to X-ray irradiation at room temperature, the frozen crystals at 100 K showed no apparent decay during the intensity measurement.

机译：UvrB DNA切除修复酶,从栖热菌属酸奶有助于HB8结晶了使用硫酸锂扩散方法沉淀剂和beta-octylglucoside作为添加剂。空间群P3121或P3221,晶胞维a = b = 136.0和c = 108.1。水晶是最有可能包含一个UvrB蛋白质在不对称单位Vm的价值3.8 A3 Da-1。超出2.9决议。对x射线照射在房间吗在100 K温度,冷冻结晶在强度没有明显衰减测量。

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来源
《Acta crystallographica.Section D. Biological crystallography》 |1999年第3期|704-705|共2页
作者
Shibata A; Sugahara M; Masui RKato RKuramitsu SFukuyama KNakagawa N;
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Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.;

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正文语种英语
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Excision Repair; Thermus thermophilus; CrystallographycrystalsX-ray diffractionintensity measurementCrystallizationProtein ConformationDNA Repaircell dimensionslithium sulfatesX-raysx ray irradiation;

机译：切除修复;栖热菌属酸奶;CrystallographycrystalsX-raydiffractionintensitymeasurementCrystallizationProtein ConformationDNARepaircell dimensionslithium sulfatesX-raysx雷辐照;

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Crystallization and preliminary X-ray diffraction studies of a DNA excision repair enzyme, UvrB, from Thermus thermophilus HB8.

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