Crystallization and preliminary X-ray diffraction studies of perchloric acid soluble protein (PSP) from rat liver.

Djinovic Carugo K; Oka T; Saraste M

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Crystallization and preliminary X-ray diffraction studies of perchloric acid soluble protein (PSP) from rat liver.

机译：结晶和初步的x射线衍射研究高氯酸可溶性蛋白(PSP)从大鼠肝脏。

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Perchloric acid soluble protein purified from the cytosol fraction of rat liver has been crystallized in a form suitable for high-resolution X-ray diffraction studies. Octahedral crystals reaching 0.5 mm in cross-sectional diameter were produced by the hanging-drop method using polyethylene glycol (Mr = 8 kDa) as precipitant. These crystals diffract to 2.44 A on an in-house X-ray source and to 1.8 A using a bending-magnet beamline at ESRF Grenoble. The crystals belong to the cubic space group P213 with a = 89.90 A and two molecules per asymmetric unit, as indicated from a Vm value of 2.12 A3 Da-1 and self-rotation function computation. Screening for heavy-atom derivatives identified a platinum compound and xenon that bind to the protein.

机译：高氯酸的可溶性蛋白纯化胞质部分大鼠肝脏结晶形式适合高分辨率x射线衍射研究。八面体晶体达到0.5毫米横断面直径产生的悬滴法使用聚乙二醇(先生= 8 kDa)作为沉淀剂。在内部x射线源和2.44至1.8一个使用一个偏转磁铁beamline ESRF格勒诺布尔。集团P213 = 89.90 a和两个分子不对称单位,表示从一个Vm的价值2.12 A3 Da-1和固有转动功能计算。确定了铂化合物和氙结合的蛋白质。

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《Acta crystallographica.Section D. Biological crystallography》 |1999年第3期|667-668|共2页
作者
Djinovic Carugo K; Oka T; Saraste M;
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European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69012 Heidelberg, Germany. djinovic@embl-heidelberg.de;

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正文语种英语
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perchloric acid; Protein Conformation; CrystallographyX-ray diffractionAnimalscrystalsProteinRat LiverX-raysCrystallizationPlatinum CompoundsRats;

机译：高氯酸;蛋白质构象;CrystallographyX-raydiffractionAnimalscrystalsProteinRatLiverX-raysCrystallizationPlatinum CompoundsRats;

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Crystallization and preliminary X-ray diffraction studies of perchloric acid soluble protein (PSP) from rat liver.

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