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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and X-ray diffraction data of a tRNASec acceptor-stem helix.
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Crystallization and X-ray diffraction data of a tRNASec acceptor-stem helix.

机译：结晶和x射线衍射数据tRNASec acceptor-stem螺旋。

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摘要

tRNASec is a UGA suppressor tRNA which co-translationally inserts selenocysteine into proteins. Its eight-base-pair tRNASec acceptor stem, which contains key recognition elements, was synthesized using solid-phase phosphoramidite RNA chemistry. High-resolution X-ray diffraction data were collected using synchrotron radiation under cryogenic cooling conditions. The crystals diffract to a maximal resolution of 1.8 A. X-ray diffraction data were processed to 2.4 A. tRNASec microhelix crystallizes in space group R32, with cell constants a = 47.02, b = 47.02, c = 373.03 A, alpha = beta = 90, gamma = 120 degrees. The crystals contain three RNA molecules per asymmetric unit.
机译：tRNASec是佐治亚大学抑制tRNAco-translationally将硒代半胱氨酸插入蛋白质。干细胞,其中包含关键识别元素,使用固相合成phosphoramidite吗RNA化学。使用同步辐射数据收集低温冷却的条件下。衍射最大分辨率为1.8。答:tRNASec衍射数据加工到2.4microhelix结晶在空间群R32,细胞常数= 47.02,b = 47.02, c = 373.03α=β= 90,γ= 120度。晶体包含3 RNA分子不对称单元。

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