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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Structure of the side-by-side binding of distamycin to d(GTATATAC)2.
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Structure of the side-by-side binding of distamycin to d(GTATATAC)2.

机译：并列结构绑定的distamycin d (GTATATAC) 2。

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摘要

The 2.40 A resolution crystal structure of a side-by-side binding of distamycin A molecules to a DNA octamer d(GTATATAC)2 with an extended alternating TA sequence has been determined. The unit-cell parameters are a = 29.55, b = 42.18, c = 43.38 A, beta = 96.56 degrees, space group P21, with two molecules in the asymmetric unit, in contrast to all previous side-by-side distamycin-DNA complexes which have only a single DNA strand and one drug molecule in the asymmetric unit. The structure was solved by the molecular-replacement method and refined to an R index of 21.0% using 3467 reflections [>/= 2sigma(F)]. The minor grooves of the DNA molecules bind two side-by-side antiparallel staggered distamycins spanning about five base pairs and virtually covering the entire length of the DNA. The octamer duplexes exhibit low-high alternations in the helical twist, sugar puckering and the C-O3' and O3'-P torsion angles, similar to the earlier side-by-side complexes containing inosine bases. The molecules are stacked one over the other along the ac diagonal in an infinite pseudo-continuous helical column with no lateral interactions.
机译：2.40的分辨率晶体结构并排绑定distamycin分子DNA八聚物与扩展d (GTATATAC) 2交替TA序列被确定。晶胞参数= 29.55,= 42.18 b, c= 43.38,β= 96.56度,P21空间群,与不对称单位,两个分子相比以前所有并排只有一个distamycin-DNA复合物DNA链和药物分子之一不对称单元。molecular-replacement方法和改进的R指数的21.0%使用3467反射[> / =2σ(F)]。分子绑定两个并列的反平行的交错distamycins横跨大约五基地对,几乎覆盖整个长度DNA。交替螺旋扭曲,糖皱纹和C-O3和O3 - p扭转角,类似于前面的复合物含有肌苷基地。沿着ac斜叠在另一个在一个无限pseudo-continuous螺旋列没有横向交互。

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