Expression, purification, crystallization and preliminary x-ray analysis of the kappa-carrageenase from Pseudoalteromonas carrageenovora.

Michel G; Flament D; Vernet TKloareg BDideberg OBarbeyron T

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Expression, purification, crystallization and preliminary x-ray analysis of the kappa-carrageenase from Pseudoalteromonas carrageenovora.

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Expression, purification, crystallization and preliminary x-ray analysis of the kappa-carrageenase from Pseudoalteromonas carrageenovora.

机译：表达,纯化,结晶初步的x射线分析从Pseudoalteromonas kappa-carrageenasecarrageenovora。

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A recombinant form of His-tagged kappa-carrageenase from Pseudoalteromonas carrageenovora has been expressed, purified and crystallized. Crystals have been obtained by the vapour-diffusion method using polyethylene glycol (Mr = 4000) as a precipitant. These crystals belong to the space group P212121, with unit-cell parameters a = 58.2, b = 62.8, c = 77.9 A, and diffract to 2.2 A resolution on a rotating-anode X-ray source.

机译：一种重组His-tagged从Pseudoalteromonas kappa-carrageenasecarrageenovora表达,纯化和结晶。使用聚乙二醇vapour-diffusion方法(= 4000)先生作为沉淀剂。属于空间群P212121,晶胞参数= 58.2,= 62.8 b, c = 77.9,衍射2.2旋转阳极的一项决议x射线源。

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《Acta crystallographica.Section D. Biological crystallography》 |1999年第4期|918-920|共3页
作者
Michel G; Flament D; Vernet TKloareg BDideberg OBarbeyron T;
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作者单位

Laboratoire de Cristallographie Macromoleculaire, Institut de Biologie Structurale Jean-Pierre Ebel, CNRS/CEA, 41 Avenue des Martyrs, 38027 Grenoble CEDEX 1, France.;

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X-ray analysis; X-ray diffraction; space groupsPseudoalteromonas carrageenovoraCrystallization;

机译：x射线分析、x射线衍射、空间groupsPseudoalteromonascarrageenovoraCrystallization;

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Expression, purification, crystallization and preliminary x-ray analysis of the kappa-carrageenase from Pseudoalteromonas carrageenovora.

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