A method to produce microseed stock for use in the crystallization of biological macromolecules.

Luft JR; DeTitta GT

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A method to produce microseed stock for use in the crystallization of biological macromolecules.

机译：一个方法生产microseed股票的使用生物大分子的结晶。

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A method is presented for producing a seed-stock mixture for macromolecular crystallization. A PTFE bead and micro-centrifuge tube act as mortar and pestle for pulverizing seed crystals of macromolecules. Energy for the bead's motion is supplied by a vortex mixer or an ultrasonic bath. The crushed crystal is serially diluted to prepare a seed-stock mixture of the desired concentration for crystallization. Crystals produced using both hanging-drop vapor diffusion and a capillary microbatch method show expected dilution behavior. This technique of producing seed stock is compared with traditional means and advantages over the standard protocol are demonstrated.

机译：提出了一种方法生产种子储备高分子结晶的混合物。聚四氟乙烯珠和micro-centrifuge管作为砂浆和杵磨粉种子的晶体大分子。由旋涡混合器或超声波浴。碎水晶连续稀释准备一个种子储备所需的混合物为结晶浓度。生产使用悬滴蒸汽扩散和毛细管microbatch方法显示预期稀释的行为。与传统手段和种子股票相比优势的标准协议演示。

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《Acta crystallographica.Section D. Biological crystallography》 |1999年第5期|988-993|共6页
作者
Luft JR; DeTitta GT;
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作者单位

Hauptman-Woodward Medical Research Institute, 73 High Street, Buffalo, New York 14203-1196, USA. luft@hwi.buffalo.edu;

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原文格式 PDF
正文语种英语
中图分类
关键词
Vortex Mixer; crystals; Crystallization;

机译：旋涡混合器;晶体;结晶;

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A method to produce microseed stock for use in the crystallization of biological macromolecules.

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