Isolation, purification, crystallization and preliminary X-ray analysis of beta 1-bungarotoxin from Bungarus caeruleus (Indian common krait).

Sharma S; Betzel C; Singh TPKarthikeyan S

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Isolation, purification, crystallization and preliminary X-ray analysis of beta 1-bungarotoxin from Bungarus caeruleus (Indian common krait).

【24h】

Isolation, purification, crystallization and preliminary X-ray analysis of beta 1-bungarotoxin from Bungarus caeruleus (Indian common krait).

机译：分离、纯化、结晶和初步的x射线分析β1-bungarotoxin从Bungarus山雀(印度常见的金环蛇)。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Beta-Bungarotoxin is a heterodimeric neurotoxin consisting of a phospholipase A2 subunit linked by a disulfide bond to a K+ channel binding subunit, which is a member of of the Kunitz protease-inhibitor subfamily. Purified beta1-bungarotoxin was crystallized using microdialysis techniques. The rectangular-shaped crystals are orthorhombic, space group C2221, with unit-cell dimensions a = 80.7, b = 82.5, c = 56.9 A. The crystals have a calculated Vm of 2.35 A3 Da-1 for one molecule in the asymmetric unit. This corresponds to a solvent content of 48%. X-ray intensity data were collected to 2.6 A resolution. The data set is 97.4% complete.

机译：Beta-Bungarotoxin heterodimeric神经毒素磷脂酶A2亚基组成的联系通过二硫键K +通道绑定亚基,这是一个Kunitz的成员蛋白酶抑制剂亚科。beta1-bungarotoxin结晶使用microdialysis技术。斜方晶系的晶体,空间群C2221,单胞尺寸= 80.7,b = 82.5, c =56.9。A3 Da-1一个分子的不对称单元。这对应于一个溶剂含量的48%。2.6 x射线强度数据收集决议。

著录项

来源
《Acta crystallographica.Section D. Biological crystallography》 |1999年第5期|1093-1094|共2页
作者
Sharma S; Betzel C; Singh TPKarthikeyan S;
展开▼
作者单位

Department of Biophysics, All India Institute of Medical Sciences, New Delhi 11 00 29, India.;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类
关键词
X-ray analysis; beta-Bungarotoxin; CrystallographyAnimalsDisulfide LinkageBungarus caeruleusX-raysCrystallization;

机译：x射线分析;beta-Bungarotoxin;CrystallographyAn;

相似文献

外文文献
中文文献

1. Science Letters:Preparation, crystallization and preliminary X-ray diffraction analysis of PH1948, predicted RNA methyltransferase from Pyrococcus horikoshii [J] . GAO Yong-gui, YAO Min, TANAKA Isao 浙江大学学报（英文版）（B辑：生物医学和生物技术） . 2005,第006期
2. Food Spectrum of Common Kraits(Bungarus caeruleus): An Implication for Snakebite Prevention and Snake Conservation [J] . Deb P. Pandey, Pranish Bhattarai, Ram C. Piya Journal of herpetology . 2020,第1期

机译：普通kraits的食物谱（Bungarus caeruleus）：对蛇咬伤和蛇保存的含义
3. Quantitative proteomic analysis of venom from Southern India common krait (Bungarus caeruleus) and identification of poorly immunogenic toxins by immune-profiling against commercial antivenom [J] . Patra Aparup, Chanda Abhishek, Mukherjee Ashis K. Expert review of proteomics . 2019,第1a6期

机译：来自印度南部的毒液的定量蛋白质组学分析常见的KRAIT（BUNGARUS caeruleus）和商业抗静电型免疫分析鉴定免疫原毒素
4. Food spectrum of Common Krait (Bungarus caeruleus): implications for snakebite prevention and snake conservation [J] . Pandey Deb P., Bhattarai Pranish, Piya Ram C. Clinical toxicology: the official journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists . 2018,第7期

机译：普通KRAIT的食物谱（BUNGARUS CAERULEUS）：对蛇咬预防和蛇保护的影响
5. Sequence-induced trimerization of phospholipase A2: structure of a trimeric isoform of PLA2 from common krait (Bungarus caeruleus) at 2.5 Å resolution [O] . Garima Singh, S. Gourinath, K. Saravanan, 2005

机译：磷脂酶A2的序列诱导三聚化：来自普通krait（Bungarus caeruleus）的PLA2三聚体异构体的结构分辨率为2.5Å
6. Crystallization and preliminary X-ray diffraction analysis of phospholipase A(1) isolated from hornet (Vespa basalis) venom [O] . Chou, C.C., Hou, M.H., 侯明宏 2014

机译：Crystallization and preliminary X-ray diffraction analysis of phospholipase a(1) isolated from hornet (Vespa basalis) venom

Isolation, purification, crystallization and preliminary X-ray analysis of beta 1-bungarotoxin from Bungarus caeruleus (Indian common krait).

摘要

著录项

相似文献

相关主题

期刊订阅