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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Purification of histone core octamers and 2.15 A X-ray analysis of crystals in KCl/phosphate.
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Purification of histone core octamers and 2.15 A X-ray analysis of crystals in KCl/phosphate.

机译：2.15组蛋白八聚物和核心的提纯晶体的x射线分析氯化钾/磷酸。

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摘要

Intact histone octamers, produced by a new method quickly and in bulk, were crystallized in KCl/phosphate, and the X-ray data were analysed to 2.15 A, confirming a P65 space group. This environment preserves the high-resolution structure of the octamers and will be useful for studying them with other functionally important molecules. The octamers form into left-handed superhelices hexagonally spaced by 158.65 A, having a pitch of 102.57 A with six octamers per turn. A dipotassium tetraiodo mercurate derivative had good phasing power and should prove valuable in refining the structure after molecular-replacement analysis with lower resolution coordinates; the heavy atom was isomorphously placed at a unique site between the two H3-cysteine residues in the octamer.
机译：完整的组蛋白八聚物,所产生的一种新方法迅速和散装结晶氯化钾、磷酸和x射线数据进行分析2.15,证实P65空间群。环境保护的高分辨率八聚物的结构,将是有用的研究它们与其它功能很重要分子。superhelices六角间距为158.65,有一个102.57一个有六个八聚物转弯。导数有充分的权力,应该逐步退出计划在精炼后的结构证明价值molecular-replacement分析较低决议坐标;同晶型之间的放置在一个独特的网站两个H3-cysteine八聚物残留。

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