Crystallization and preliminary X-ray diffraction analysis of haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26.

Smatanova I; Svensson LA; Takagi MMarek JNagata Y

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray diffraction analysis of haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26.

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Crystallization and preliminary X-ray diffraction analysis of haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26.

机译：结晶和初步的x射线衍射分析了卤代烷dehalogenase LinB

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Haloalkane hydrolytic dehalogenase LinB from Sphingomonas paucimobilis UT26, an enzyme which releases chloride or bromide anion from n-halogenated alkanes and has a broad range of substrate specificity, was crystallized using the hanging-drop vapour-diffusion method at 278 K. The best crystals were obtained by microseeding with a precipitant containing 18-20%(w/v) PEG 6000, 0.2 M calcium acetate and 0.1 M Tris-HCl pH 8.9. The crystals diffract to at least 1.60 A using synchrotron X-ray under cryogenic (100 K) conditions. They belong to the orthorhombic space group P21212 with unit-cell parameters a = 50.29, b = 71.70, c = 72.73 A. The asymmetric unit contains one molecule of the enzyme.

机译：卤代烷水解dehalogenase LinBSphingomonas paucimobilis UT26,一种酶释放氯或溴阴离子n-halogenated烷烃和广泛的底物特异性,是固定使用在278 K悬滴vapour-diffusion方法。最好的水晶被microseeding获得沉淀剂含有18 - 20% (w / v)挂钩6000年,乙酸钙0.2米和0.1米Tris-HCl pH8.9. 使用同步加速器x射线在低温(100 K)下条件。集团P21212晶胞参数= 50.29,b = 71.70, c = 72.73。包含一个分子的酶。

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《Acta crystallographica.Section D. Biological crystallography》 |1999年第6期|1231-1233|共3页
作者
Smatanova I; Svensson LA; Takagi MMarek JNagata Y;
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作者单位

Laboratory of Biomolecular Structure and Dynamics and Department of Inorganic Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, CZ 611 37 Brno, Czech Republic.;

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正文语种英语
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Pseudomonas paucimobilis; Protein Conformation; CrystallographySubstrate Specificityhaloalkane dehalogenaseX-ray diffraction analysisX-raysCrystallization;

机译：假单胞菌paucimobilis;蛋白质构象;CrystallographySubstrate衍射analysisX-raysCrystallization;

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Crystallization and preliminary X-ray diffraction analysis of haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26.

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