Production, crystallization and preliminary X-ray analysis of the human integrin alpha1 I domain.

Salminen TA; Kankare J; Kapyla JHeino JJohnson MSNymalm Y

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Production, crystallization and preliminary X-ray analysis of the human integrin alpha1 I domain.

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Production, crystallization and preliminary X-ray analysis of the human integrin alpha1 I domain.

机译：生产、结晶和初步的x射线分析人类的整合素α1我的域。

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Integrin alpha1beta1 is one of the main collagen receptors in many cell types. A fast large-scale production, purification and crystallization method for the integrin alpha1 I domain is reported here. The alpha1 I domain was crystallized using the vapour-diffusion method with a reservoir solution containing a mixture of PEG 4000, sodium acetate, glycerol and Tris-HCl buffer. The crystals belong to the C2 space group, with unit-cell parameters a = 74.5, b = 81.9, c = 37.3 A, alpha = gamma = 90.0, beta = 90.8 degrees. The crystals diffract to 2.0 A and a 94.2% complete data set to 2.2 A has been collected from a single crystal with an Rmerge of 5.8%.

机译：整合素alpha1beta1胶原蛋白的主要成分之一在许多类型的细胞受体。生产、纯化和结晶方法整合素α1我的域报告在这里。使用vapour-diffusion结晶的方法与储层的解决方案包含的混合物4000年挂钩,醋酸钠,甘油和Tris-HCl缓冲区。b组,晶胞参数= 74.5,=81.9、37.3 c =α=γ= 90.0,β=90.8度。完整的数据设置为2.2 94.2%从单晶的Rmerge收集5.8%.

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《Acta crystallographica.Section D. Biological crystallography》 |1999年第7期|1365-1367|共3页
作者
Salminen TA; Kankare J; Kapyla JHeino JJohnson MSNymalm Y;
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Department of Biochemistry and Pharmacy, Abo Akademi University, PO Box 66, FIN-20521 Turku, Finland. tiina.salminen@abo.fi;

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正文语种英语
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Human; Protein Conformation; X-ray analysisCollagen ReceptorsCrystallographyIntegrin alpha1Integrin alpha1beta1antigensspace groupsX-raysCrystallization;

机译：人类;蛋白质构象;x射线analysisCollagenReceptorsCrystallographyIntegrin alpha1IntegringroupsX-raysCrystallization;

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Production, crystallization and preliminary X-ray analysis of the human integrin alpha1 I domain.

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