Crystallization and preliminary X-ray analysis of wild-type and V103L mutant Myb R2 DNA-binding domain.

Tahirov TH; Uedaira H; Sarai AOgata KMorii H

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Crystallization and preliminary X-ray analysis of wild-type and V103L mutant Myb R2 DNA-binding domain.

机译：结晶和初步的x射线分析野生型和突变Myb V103L R2 dna结合蛋白域。

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The R2 subdomain of the mouse c-Myb DNA-binding domain and its V103L mutant have been crystallized by the vapour-diffusion method using highly concentrated sodium citrate at pH 6.8 as a precipitant. Using ammonium sulfate as precipitant in MES buffer only produced crystals for the mutant R2. All crystals are isomorphous and belong to space group P212121. The unit-cell dimensions for wild-type R2 crystals grown from sodium citrate precipitant are a = 28.83, b = 40.18, c = 49.23 A. Crystals contain one R2 molecule per asymmetric unit. They are stable during 3 d exposure to X-rays and diffract to 1.37-1.45 A resolution.

机译：鼠标c-Myb dna结合蛋白的R2子域名域及其V103L突变vapour-diffusion结晶的方法使用高度集中的柠檬酸钠在pH值为6.8沉淀剂。沉淀剂在MES缓冲区只有产生晶体R2的突变。和属于空间P212121组。尺寸为野生型R2晶体生长柠檬酸钠沉淀剂= 28.83,=40.18, c = 49.23。分子/不对称单位。在3 d接触x射线和衍射1.37 - -1.45一项决议。

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《Acta crystallographica.Section D. Biological crystallography》 |1999年第7期|1345-1347|共3页
作者
Tahirov TH; Uedaira H; Sarai AOgata KMorii H;
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Kanagawa Academy of Science and Technology (KAST), 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan.;

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正文语种英语
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sodium citrate; Protein Conformation; MutantsX-ray analysisCrystallographyMutationAnimalscrystalsX-raysCrystallizationisomorphous substitution;

机译：柠檬酸钠、蛋白质构象MutantsX-ray;

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Crystallization and preliminary X-ray analysis of wild-type and V103L mutant Myb R2 DNA-binding domain.

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