Crystallization and initial X-ray analysis of rabbit mature sterol carrier protein 2.

Choinowski T; Maderegger B; Winterhalter KHHauser HPiontek KDyer JH

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Crystallization and initial X-ray analysis of rabbit mature sterol carrier protein 2.

机译：结晶和初始x射线分析兔子成熟甾醇载体蛋白2。

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Sterol carrier protein 2 (SCP2) is a basic intracellular protein which facilitates the in vitro intermembrane transfer of cholesterol, phospholipids and glycolipids. SCP2 was expressed in Escherichia coli, purified to apparent electrophoretic homogeneity and crystallized. Single crystals were obtained by hanging-drop vapour diffusion using ammonium sulfate as precipitant. These crystals belong to space group P4(1)2(1)2 or its enantiomorph, with unit-cell parameters a = b = 57.5, c = 86.5 A, and have one molecule in the crystallographic asymmetric unit. Intensity data to 1.8 A resolution were collected from native SCP2 crystals using synchrotron radiation, were processed and scaled with an R(linear) = 4.9%.

机译：甾醇载体蛋白2 (SCP2)是一个基本的细胞内蛋白质起到促进作用体外膜间隙的胆固醇,磷脂和糖脂。在大肠杆菌中,纯化明显电泳同质性和结晶。单晶是通过悬滴蒸汽扩散使用硫酸铵沉淀剂。P4(1) 2(1) 2或其对映体,与单胞参数a = b = 57.5, c = 86.5,和有一个分子晶体的不对称单元。1.8强度数据收集的决议从本地SCP2晶体使用同步加速器辐射处理和缩放R(线性)= 4 . 9%。

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《Acta crystallographica.Section D. Biological crystallography》 |1999年第8期|1478-1480|共3页
作者
Choinowski T; Maderegger B; Winterhalter KHHauser HPiontek KDyer JH;
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Institut fur Biochemie, Swiss Federal Institute of Technology (ETH), Universitatstrasse 16, CH-8092 Zurich, Switzerland.;

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正文语种英语
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Molecular weights; X-ray analysis; CrystallographyAnimalsrabbitsX-rayssterol carrier proteinCrystallization;

机译：分子量、x射线分析、CrystallographyAn;

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Crystallization and initial X-ray analysis of rabbit mature sterol carrier protein 2.

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