Crystallization and preliminary X-ray diffraction studies of mammalian purple acid phosphatase.

Guddat LW; Hume D; de Jersey JHamilton SMartin JLMcAlpine AS

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Crystallization and preliminary X-ray diffraction studies of mammalian purple acid phosphatase.

机译：结晶和初步的x射线衍射研究哺乳动物紫色酸性磷酸酶。

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The oxidized form of purple acid phosphatase from pig allantoic fluid has been crystallized in the presence of phosphate using the hanging-drop technique. The crystals belong to the space group P2(1)2(1)2(1) and have unit-cell parameters a = 66.8, b = 70.3, c = 78.7 A. Diffraction data collected from a cryocooled crystal using a conventional X-ray source extend to 1.55 A resolution. A knowledge of the three-dimensional structure of mammalian purple acid phosphatase will aid in understanding the substrate specificity of the enzyme and will be important in the rational design of inhibitors, with potential in the treatment of bone diseases.

机译：紫色酸性磷酸酶的氧化形式猪尿囊液的结晶使用悬滴磷酸的存在技术。P2(1) 2(1) 2(1),有晶胞参数=66.8, b = 70.3, c = 78.7。收集从cryocooled水晶使用传统的x射线源扩展到1.55决议。哺乳动物的紫色酸性磷酸酶的结构帮助理解衬底吗特异性的酶,将是重要的设计合理的抑制剂,骨骼疾病的潜在治疗。

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《Acta crystallographica.Section D. Biological crystallography》 |1999年第8期|1462-1464|共3页
作者
Guddat LW; Hume D; de Jersey JHamilton SMartin JLMcAlpine AS;
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Department of Biochemistry, The University of Queensland, St Lucia, Queensland 4072, Australia. guddat@biosci.uq.edu.au;

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正文语种英语
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purple acid phosphatase; rational design; CrystallographyAnimalsX-ray diffractionSubstrate SpecificityX-raysFemaleacid phosphataseCrystallizationPregnancyallantoic fluid;

机译：紫色酸性磷酸酶;理性设计;CrystallographyAnimalsX-raydiffractionSubstrate SpecificityX-raysFemaleacidphosphataseCrystallizationPregnancyallantoic流体;

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Crystallization and preliminary X-ray diffraction studies of mammalian purple acid phosphatase.

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