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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray analysis of human rhinovirus serotype 2 (HRV2).
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Crystallization and preliminary X-ray analysis of human rhinovirus serotype 2 (HRV2).

机译：结晶和初步的x射线分析人类鼻病毒血清型2 (HRV2)。

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摘要

Human rhinoviruses, the major cause of mild recurrent infections of the upper respiratory tract, are small icosahedral particles. Over 100 different serotypes have been identified. The majority (91 serotypes) use intercellular adhesion molecule 1 as the cell-attachment site; ten serotypes (the minor group) bind to members of the low-density lipoprotein receptor. Three different crystal forms of the minor-group human rhinovirus serotype 2 (HRV2) were obtained by the hanging-drop vapour-diffusion technique using ammonium sulfate and sodium/potassium phosphate as precipitants. Monoclinic crystals, space group P2(1), diffracted at least to 2.8 A resolution, and two complete virus particles were located in the crystal asymmetric unit. A second type of crystals had a compact cubic like morphology and diffracted beyond 2.5 A resolution. These crystals belong to a primitive orthorhombic space group, with unit-cell parameters a = 309.3, b = 353.5, c = 759.6 A, and contain one virus particle in the asymmetric unit. A third type of crystals, with a prismatic shape and belonging to space group I222, was also obtained under similar crystallization conditions. These latter crystals, with unit-cell parameters a = 308.7, b = 352.2, c = 380.5 A, diffracted to high resolution (beyond 1.8 A) and contained 15 protomers per asymmetric unit; this requires that three perpendicular crystal twofold axes coincide with three of the viral particle's dyad axes.
机译：人类鼻病毒,轻微的主要原因反复上呼吸道感染束,小二十面体粒子。不同的血清型有被确认。多数(91种血清型)使用细胞间粘附分子1 cell-attachment网站;十种血清型(小组)绑定到成员低密度脂蛋白的受体。不同的晶体形式的minor-group人类鼻病毒血清型2 (HRV2)获得的悬滴vapour-diffusion技术使用硫酸铵和钠/钾磷酸盐作为沉淀剂。P2(1)衍射至少2.8一项决议,和两个完整的病毒粒子位于水晶不对称单位。水晶立方形态学和紧凑衍射分辨率2.5之外。水晶属于原始斜方晶系的空间b组,晶胞参数= 309.3,=353.5、c = 759.6和包含一个病毒粒子在不对称单位。晶体,通过棱镜的形状和归属感空间群I222,也获得了相似的结晶条件。晶体晶胞参数= 308.7,b= 352.2, c = 380.5,衍射到高决议(超过1.8),包含15原体/不对称单位;三个垂直晶体两个坐标轴一致有三个病毒粒子的二分体轴。

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