Derivative manipulation in the structure solution of the integral membrane LH2 complex.

Prince SM; Freer AA; Papiz MZLawless AMCogdell RJIsaacs NWMcDermott G

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Derivative manipulation in the structure solution of the integral membrane LH2 complex.

机译：导数处理结构的解决方案膜LH2复杂的积分。

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The structure of the peripheral light-harvesting complex from Rhodopseudomonas acidophila strain 10050 was determined by multiple isomorphous replacement methods. The derivatization of the crystals was augmented by the addition of a backsoaking stage. The soak/backsoaked data comparison had greater isomorphism and showed simpler Patterson maps than the standard native/soak comparison. Amplitudes from the derivatized then backsoaked crystals and from the derivatized crystals were compared in order to extract a subset of heavy-atom sites. Using this information, the full array of sites were found from a derivative/native comparison, eventually leading to excellent electron-density maps.

机译：外围聚光的结构复杂的从Rhodopseudomonas acidophila压力10050年是由多个同形替代方法。晶体被添加增强backsoaking阶段。比较有更大的同构和显示简单的帕特森地图比标准的本地/浸泡比较。derivatized然后backsoaked晶体的为了derivatized晶体进行了比较提取重原子站点的一个子集。信息,网站被发现的全套设备从衍生品/本地比较,最终导致优秀的电子密度图。

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来源
《Acta crystallographica.Section D. Biological crystallography》 |1999年第8期|1428-1431|共4页
作者
Prince SM; Freer AA; Papiz MZLawless AMCogdell RJIsaacs NWMcDermott G;
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作者单位

Department of Chemistry, University of Glasgow, Glasgow G12 8QQ, Scotland.;

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原文格式 PDF
正文语种英语
中图分类
关键词
Protein Structure; Crystallography; crystalsX-raysPhotosynthetic Reaction CentersCrystallizationData comparison;

机译：蛋白质结构,结晶学;crystalsX-raysPh;

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Derivative manipulation in the structure solution of the integral membrane LH2 complex.

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