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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Is single-wavelength anomalous scattering sufficient for solving phases? A comparison of different methods for a 2.1 A structure solution.
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Is single-wavelength anomalous scattering sufficient for solving phases? A comparison of different methods for a 2.1 A structure solution.

机译:是单波长反常散射足够的解决阶段?2.1不同的方法对结构的解决方案。

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摘要

The structure of rusticyanin is the largest unknown structure (M(r) = 16.8 kDa) which has been recently solved by the direct-methods approach using only single-wavelength anomalous scattering (SAS) data from the native protein [Harvey et al. (1998). Acta Cryst. D54, 629-635]. Here, the results of the Sim distribution approach [Hendrickson & Teeter (1981). Nature (London), 290, 107-113] and of the CCP4 procedure MLPHARE [Collaborative Computational Project, Number 4 (1994). Acta Cryst. D50, 760-763] are compared with those from direct methods. Analysis against the final refined model shows that direct methods produced significantly better phases (average phase error 56 degrees ) and therefore significantly better electron-density maps than the Sim distribution and MLPHARE approaches (average phase error was around 63 degrees in both cases).
机译:rusticyanin是最大的结构未知的结构(M (r) = 16.8 kDa)最近由直接方法解决方法只使用单波长反常散射(SAS)数据从本地的蛋白质黑哈维科幻片阿尔。(1998)。在这里,Sim分布的结果方法[Hendrickson &摇摇欲坠(1981)。(伦敦),290年,107 - 113]和CCP4过程MLPHARE[协作计算项目,4号(1994)。相比与直接的方法。对最终的细化模型显示直接方法产生更好的阶段(平均相位误差56度),因此更好的电子密度比地图Sim分布和MLPHARE方法(平均相位误差约为63度这两种情况下)。

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