Preparation and preliminary X-ray diffraction studies of a new crystal form of L-asparaginase from Escherichia coli.

Polikarpov I; Abrahao-Neto J; de Oliveira RT

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Preparation and preliminary X-ray diffraction studies of a new crystal form of L-asparaginase from Escherichia coli.

机译：准备和初步的x射线衍射研究一个新的水晶L-asparaginase形式从大肠杆菌。

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L-Asparaginase is an enzyme which hydrolyzes asparagine to produce aspartic acid and ammonia. It is an effective chemotherapeutic drug, especially in the treatment of acute lymphoblastic leukaemia in children. The enzyme from Escherichia coli was crystallized in a new crystal form with space group C2, unit-cell parameters a = 76.3 (0), b = 134.6 (2), c = 64.8 (7) A, beta = 110.5 (1) degrees and a dimer in the asymmetric unit. Synchrotron-radiation diffraction data have been collected to 1.95 A resolution.

机译：L-Asparaginase是水解酶天冬酰胺生成天冬氨酸和氨。它是一种有效的化疗药物,尤其是在治疗急性淋巴细胞白血病儿童。从大肠杆菌是一种新的结晶水晶形式与空间群C2,晶胞参数= 76.3 (0),b = 134.6 (2), c = 64.8(7),β= 110.5(1)度和二聚体不对称单元。衍射数据已经收集了1.95决议。

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《Acta crystallographica.Section D. Biological crystallography》 |1999年第9期|1616-1617|共2页
作者
Polikarpov I; Abrahao-Neto J; de Oliveira RT;
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Laboratorio Nacional de Luz Sincrotron, Caixa Postal 6192, CEP 13083-970, Campinas SP, Brazil. igor@lnls.br;

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正文语种英语
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Escherichia coli; preparation; Precursor Cell Lymphoblastic Leukemia-LymphomaX-ray diffractionAsparaginaseAspartic Acidcrystal outlineCrystallizationAsparagine;

机译：大肠杆菌;制备;细胞前体淋巴细胞Leukemia-LymphomaX-raydiffractionAsparaginaseAspartic AcidcrystaloutlineCrystallizationAsparagine;

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Preparation and preliminary X-ray diffraction studies of a new crystal form of L-asparaginase from Escherichia coli.

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