Crystallization and preliminary X-ray crystallographic studies of the D2 region of the skeletal muscle ryanodine receptor.

Kim S; Kim DH; Eom SHShin DW

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Crystallization and preliminary X-ray crystallographic studies of the D2 region of the skeletal muscle ryanodine receptor.

机译：结晶和初步的x射线晶体的研究D2的区域骨骼肌阿诺定受体。

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The N-terminal portion (amino acids 1303-1367) of the type 1 ryanodine receptor D2 region is thought to be critical for excitation-contraction coupling in skeletal muscle. A segment of the D2 region (amino acids 1317-1355) was expressed as a glutathione S-transferase fusion protein (GST-D2) and then crystallized at room temperature using ammonium sulfate as precipitant. Using a newly developed cryo-soaking method, complete native data sets were measured to a resolution of 2.2 A using synchrotron radiation. The crystal was found to be hexagonal, belonging to space group P6(3)22, with unit-cell parameters a = b = 116.1, c = 77.9 A.

机译：氨基酸氨基部分(1303 - 1367)1型阿诺定受体D2地区认为是兴奋收缩的关键在骨骼肌耦合。氨基酸区域(1317 - 1355)被表示为一个谷胱甘肽S-transferase融合蛋白(GST-D2)然后固定在室温下使用硫酸铵沉淀剂。cryo-soaking开发方法,完成本地2.2数据集测量的分辨率使用同步辐射。发现是六角形的,属于空间群P6(3) 22日,晶胞参数a = b = 116.1,c = 77.9。

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《Acta crystallographica.Section D. Biological crystallography》 |1999年第9期|1601-1603|共3页
作者
Kim S; Kim DH; Eom SHShin DW;
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Department of Life Science, Kwangju Institute of Science and Technology, Kwangju 500-712, South Korea.;

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正文语种英语
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Skeletal muscle; muscle fibers; RYR1 genedodecyl sulfate sodiumAnimalsrabbitsmusclesExcitation Contraction CouplingRyanodine Receptor Calcium Release ChannelCrystallizationpolyacrylamide gelsElectrophoresis;

机译：骨骼肌肌纤维;RYR1 genedodecyl硫酸sodiumAnimalsrabbitsmusclesExcitation收缩CouplingRyanodine受体钙释放ChannelCrystallizationpolyacrylamidegelsElectrophoresis;

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Crystallization and preliminary X-ray crystallographic studies of the D2 region of the skeletal muscle ryanodine receptor.

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