Crystallization and preliminary X-ray analysis of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (tyrosine inhibitable) from Saccharomyces cerevisiae.

Schneider TR; Braus GH; Hartmann M

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray analysis of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (tyrosine inhibitable) from Saccharomyces cerevisiae.

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Crystallization and preliminary X-ray analysis of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (tyrosine inhibitable) from Saccharomyces cerevisiae.

机译：结晶和初步的x射线分析3-deoxy-D-arabino-heptulosonate-7-phosphate合酶(酪氨酸的制约)酿酒酵母。

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3-Deoxy-D-arabino-heptulosonate-7-phosphate synthase (E.C. 4.1.2.15) catalyses the first step in the biosynthesis of aromatic amino acids: the condensation of phophoenolpyruvate and erythrose 4-phosphate to 3-deoxy-D-arabino-heptulosonate-7-phosphate. Diffraction-quality crystals of the tyrosine-inhibitable form of the enzyme from Saccharomyces cerevisiae have been obtained by the hanging-drop vapour-diffusion method in the presence of polyethylene glycol. The crystals belong to the triclinic space group P1, with unit-cell parameters a = 81.5, b = 94.0, c = 104.6 A, alpha = 65.5, beta = 85.2, gamma = 75.0 degrees, and can be flash-cooled using glycerol as a cryoprotectant. A data set to 2.3 A has been collected at 120 K.

机译：3-Deoxy-D-arabino-heptulosonate-7-phosphate合酶(提到过4.1.2.15)催化作用的第一步在芳香族氨基酸的生物合成冷凝phophoenolpyruvate和赤藓糖4-phosphate来3-deoxy-D-arabino-heptulosonate-7-phosphate。Diffraction-quality晶体的tyrosine-inhibitable形成的酶酿酒酵母已经获得的的悬滴vapour-diffusion方法聚乙二醇的存在。属于三斜晶系的空间群P1晶胞参数= 81.5,b = 94.0, c =104.6,α= 65.5,β= 85.2,γ= 75.0度,可以使用甘油flash-cooled作为冷冻保护剂。收集了在120 K。

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《Acta crystallographica.Section D. Biological crystallography》 |1999年第9期|1586-1588|共3页
作者
Schneider TR; Braus GH; Hartmann M;
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Lehrstuhl fur Strukturchemie, Universtitat Gottingen, Tammannstrasse 4, D-37077 Gottingen, Germany. trs@shelx.uni-ac.gwdg.de;

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X-ray analysis; erythrose 4-phosphate; Saccharomyces cerevisiaeX-ray diffraction3-deoxy-D-arabino-heptulosonate-7-phosphateTyrosine Metabolism PathwayCrystallization;

机译：x射线分析;赤藓糖4-phosphate;酿酒cerevisiaeX-ray;

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Crystallization and preliminary X-ray analysis of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (tyrosine inhibitable) from Saccharomyces cerevisiae.

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