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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization of truncated human apolipoprotein A-I in a novel conformation.
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Crystallization of truncated human apolipoprotein A-I in a novel conformation.

机译：结晶的截断人类载脂蛋白ⅰ在小说构象。

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摘要

The crystallization of recombinant human apolipoprotein A-I (apo A-I), the major protein component of high-density lipoprotein, in a new crystal form is described. The fragment crystallized, residues 44-243 of native apo A-I [apo Delta(1--43)A-I], is very similar to intact native apo A-I in its ability to bind lipid, to be incorporated into high-density lipoproteins and to activate lecithin-cholesterol acyl transferase. Apo Delta(1-43)A-I crystallizes, in the presence of beta-D-octylglucopyranoside, in space group I222 or I2(1)2(1)2(1), with unit-cell parameters a = 37. 11, b = 123.62, c = 164.65 A and a diffraction limit of 3.2 A. These form II crystals grow under conditions of significantly lower ionic strength than the original form I crystals (space group P2(1)2(1)2(1), a = 97.47, b = 113.87, c = 196.19 A, diffraction limit 3.0 A). Packing arguments show that the unusual open conformation of apo Delta(1-43)A-I found in the form I crystals cannot be packed into the smaller oddly proportioned form II unit cell. Monomeric apo Delta(1-43)A-I, as either a four-helix bundle ( approximately 75 x 30 x 30 A) or an extended helical rod (approximately 150 x 20 x 20 A), can be packed into the form II unit cell. It is concluded, therefore, that apo Delta(1-43)A-I may have crystallized in one of these distinct conformations in the form II crystals.
机译：重组人类的结晶载脂蛋白-ⅰ(apo -ⅰ)的主要蛋白质组件的高密度脂蛋白,在一个新的水晶形式描述。结晶,残留44 - 243的本地apo -ⅰ(apoδ(1 - 43)-ⅰ),非常类似于完好无损本机apo -ⅰ的脂质结合的能力,被纳入高密度脂蛋白和激活lecithin-cholesterol酰基转移酶。beta-D-octylglucopyranoside的存在,空间群I222或I2(1) 2(1) 2(1),与单胞参数= 37。和3.2的衍射极限。晶体生长的条件下显著我低离子强度比原来的形式晶体的空间群P2 (1) 2 (1) 2 (1) = 97.47, b= 113.87, c = 196.19, 3.0衍射极限)。包装参数表明,不同寻常的开放构象的δ-ⅰ(1)中发现的我水晶不能挤进小奇怪的是第二单元细胞成比例的形式。apoδ-ⅰ(1),作为一个四螺旋包(大约30 75 x 30 x)或一个扩展螺旋杆(大约150 x 20 x 20)被挤到第二单元细胞形式。因此得出结论,apoδ(1)ⅰ的可能有其中一个明显的结晶吗构象形式II晶体。

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