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Butanol production by Clostridium acetobutylicum DSMZ 792 from cassava starch

机译:丁醇生产acetobutylicum梭状芽胞杆菌从木薯淀粉DSMZ 792

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Abstract Cassava cultivation is cheap, with potential development under different climatic conditions. It is cultivated mainly in South America, Asia, and Africa. Cassava starch is an attractive feedstock, and has been employed as carbon source for production of butanol given its low cost and wide availability. In this sense, this study aimed to evaluate the production of butanol by Clostridium acetobutylicum DSMZ 792 using cassava starch as a substrate, applying Design of Experiments (DoE). Key parameters reported in the literature, such as pH and substrate concentration, were used in the evaluation trials by DoE using Rotatable Central Composite Design (RCCD) on butanol production during fermentation of cassava starch. This evaluation employed RCCD, which is a factorial scheme of treatment (22) considering four factorial design points (T1-T4), four axial points (T5-T8), and three replications on the central points (T9-T11), totaling 11 experiments. Fermentation was conducted in batch mode in 500?mL flasks, containing effective reaction volume of 300?mL. Concentrations of butanol, acetic and butyric acids were separated and analyzed by High-Performance Liquid Chromatography (HPLC) using a column (300?mm?×?7.8?mm). Data were collected and analyzed using Class-VP software.?The RCCD indicated that the highest butanol production is achieved in tests performed with higher concentration of the glucose, i.e., 50.0?g L?1 and with initial pH 5.7, resulting in a butanol concentration of 4.37?g L?1. The results showed that butanol production was dependent on butyric acid re-assimilation. DoE is a powerful tool for obtaining the optimum butanol concentration for determined operation condition.
机译:抽象的木薯种植便宜,潜在的发展在不同的气候条件。美国、亚洲和非洲。有吸引力的原料,被雇佣为碳源对丁醇的生产低成本和广泛的可用性。本研究旨在评估的生产丁醇的梭状芽胞杆菌acetobutylicum DSMZ 792利用木薯淀粉作为基质,应用实验设计(DoE)。在文献中报道,如pH值和底物浓度,被使用的由能源部利用旋转中央评价试验综合设计(RCCD)丁醇生产在发酵木薯淀粉。评价采用RCCD,阶乘治疗方案(22)考虑四个析因设计点(T1-T4)、4轴点(T5-T8)和三个复制中央点(T9-T11),共计11个实验。发酵是在批处理模式进行的500?量300 ?毫升。醋酸和丁酸分离分析了高效液相色谱使用一列(300毫米?×7.8 ?毫米)。分析了使用Class-VP软件。表明,丁醇产量最高在测试中表现较高的实现葡萄糖的浓度,即50.0 ?和初始pH值5.7,导致丁醇浓度的4.37 ?丁醇生产依赖于丁酸re-assimilation。获得最优丁醇浓度确定操作条件。

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