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Microbial diversity analysis of Hudiara Drain wastewater using culture-independent and culture-dependent approaches

机译：微生物多样性分析Hudiara流失废水利用文化无关,文化相关的方法

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Abstract Hudaira Drain of Pakistan is one of the largest storm water drains which is a potential threat to the environment and biodiversity. It is considered as the second largest water polluting body in Pakistan. The major polluting contributors are toxic industrial wastes, chemical fertilizers and poultry or animal waste. The objective of this study was to analyze the microbial diversity of wastewater from Hudiara Drain using culture-independent and culture-dependent techniques. For culture-independent analysis, the 16S rRNA gene-based Illumina sequencing technique was used. Metagenomic sequence analysis revealed a total of 20 different phyla in the source drain water samples. Bacterial phyla Actinobacteria, Proteobacteria, Bacteroidetes, Firmicutes, Acidobacteria, Chloroflexi, Planctomycetes, and Fusobacteria and archaeal phylum Thaumarchaeota were commonly identified from all the wastewater samples. A total of 282 genera of bacteria and archaea were identified in this study, with Bacillus, Nitrospira, Nitrososphaera, Gemmatimonas, Pseudomonas, Stenotrophomonas, Myxococcus, Azospirilum, Skermanella, Nitrososphaera, Pontibacter and Aquicella being the dominant genera. Using culture-dependent techniques, 32 bacterial strains were identified and characterized for heavy metal and antibiotic resistance. The common genera were Bacillus, Aeromonas and Enterobacter. These results suggest that the microbial diversity from Hudiara Drain wastewater samples is a potential source of clinically and industrially important bacterial and archaeal strains.
机译：文摘Hudaira排水的巴基斯坦是其中一个是一个潜在的最大的雨水下水道对环境和生物多样性的威胁。作为第二大污染水在巴基斯坦的身体。贡献者是有毒的工业废物,化肥和家禽或动物粪便。本研究的目的是分析从Hudiara废水的微生物多样性排水使用文化无关,文化相关的技术。分析文化无关,16 s rRNA基于基因的Illumina公司测序技术使用。共有20个不同类群在源流失水样本。变形菌门、拟杆菌、厚壁菌门,Acidobacteria、Chloroflexi Planctomycetes,Fusobacteria和古门Thaumarchaeota从所有的废水通常是确定吗样本。古生菌被确定在这项研究中,Nitrospira、Nitrososphaera、芽孢杆菌Stenotrophomonas Gemmatimonas,假单胞菌,Myxococcus, Azospirilum、SkermanellaNitrososphaera, Pontibacter Aquicella占主导地位的属。技术,32个菌株被确定特征和重金属和抗生素阻力。气单胞菌属、肠杆菌属。的微生物多样性Hudiara流失废水样品的一个潜在来源临床和工业重要的细菌和古细菌菌株。

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