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>Assessment and characterization of poly(3-hydroxybutyrate) accumulated by endophytic bacterium Exiguobacterium acetylicum BNL 103 from oleaginous plant Brassica napus L.
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Assessment and characterization of poly(3-hydroxybutyrate) accumulated by endophytic bacterium Exiguobacterium acetylicum BNL 103 from oleaginous plant Brassica napus L.
Abstract Endosphere, the internal environment of plants is colonized by diverse and complex endophytic microbial communities that completely or partially pass their life events inside plants. These endogenously coexisting bacterial population of plants have been well analysed for the production of wide diversity of natural compounds including the biopolyesters, polyhydroxyalkanoates (PHAs). Oleaginous plants, the fruits and seeds of which are utilized for production and extraction of oil are no exception and harbour a large array of endophytic bacteria in their internal tissues. In this study, an attempt has been made to evaluate the prospects of the bacterial endophytes of oleaginous plant, Brassica napus L. for the biosynthesis and intracellular deposition of poly(3-hydroxybutyrate) [P(3HB)], the widely known PHA. About 82.8% of total endophytic bacterial isolates showed accumulation of P(3HB) during in vitro growth in chemically defined medium under batch cultivation. The most potent bacterium, Exiguobacterium acetylicum BNL 103 (GenBank accession number KX458037, MCC accession number 3434) endophytic to leaves of B. napus L. produced P(3HB) which accounted for about 55.4% of the dry cell mass under batch cultivation. The accumulated polyester was extracted and purified from the dry cell mass following standard methods and its identity was confirmed by the ultraviolet, Fourier-transform infrared (FTIR), proton (1H) and carbon (13C) nuclear magnetic resonance (NMR) spectroscopic analysis.
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