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Enhanced ciprofloxacin removal by plant growth-promoting Microbacterium sp. WHC1 in presence of Eichhornia crassipes root exudates

机译:增强环丙沙星去除植物刺激小细菌属sp。WHC1存在Eichhornia凤眼莲根系分泌物

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Abstract Antibiotics are released undegraded or partially degraded in the environment which renders them difficult for bioremediation, therefore creating a major threat to antibiotic pollution. Ciprofloxacin (CIP) is a fluoroquinolone that is highly persistent and refractory to degradation. The current study aims to isolate and characterize the CIP resistant, rhizospheric bacteria from Eichhornia crassipes, to assess the effect of root exudates on CIP removal. Also, the plant growth-promoting (PGP) traits of the isolated bacteria were evaluated in the absence and presence of CIP. The enrichment technique in M9 minimal media was used for the isolation and bacteria was identified as Microbacterium sp. WHC1. E.crassipes was able to remove 78% of CIP from Hoagland media. However, the maximum removal was seen in presence of the root exudates and bacterial isolate WHC1 (98%). In-vitro screening of the bacteria was done for studying the PGP traits. Microbacterium sp. WHC1 showed positive results for ammonia and Indole-3-acetic acid (IAA) production, whereas negative results were obtained for hydrogen cyanide (HCN) production and phosphorous (P) solubilization. The current study findings can help in understanding the interactions between the rhizoplane bacteria and root exudates which helps in the decontamination of pollutants. PGP activities of these isolates could be a strategy to improve large scale polluted sites using green and eco-friendly technology.
机译:摘要抗生素未经碰撞的或公布部分退化的环境使他们难以生物降解,因此创建一个主要威胁抗生素污染。高度持久和氟喹诺酮类耐火材料退化。隔离并描述CIP耐药,根际细菌Eichhornia凤眼莲,评估CIP根系分泌物的影响去除。特征的孤立的细菌进行了评估没有和CIP的存在。技术在M9媒体用于最小被确认为隔离和细菌小细菌属WHC1 sp。把78%的CIP从霍格兰媒体。除最大的存在根系分泌物和细菌隔离WHC1(98%)。体外筛选细菌完成的研究了PGP特征。对氨和显示积极的结果Indole-3-acetic酸(IAA)生产,而氢的负面结果氰化物(HCN)生产和磷(P)增溶。帮助理解之间的交互rhizoplane细菌和根分泌物可以帮助净化污染物。这些隔离可能是战略的活动提高大型污染的网站使用绿色和环保技术。

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