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首页> 外文期刊>Environmental Sustainability >Evaluation of selenium biofortification strategies in Phaseolus vulgaris through selenocysteine methyltransferase gene expression
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Evaluation of selenium biofortification strategies in Phaseolus vulgaris through selenocysteine methyltransferase gene expression

机译：评估硒生物强化策略通过硒代半胱氨酸在菜豆甲基转移酶基因表达

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摘要

Abstract Plant growth promoting selenorhizobacteria have the multi-capacity to colonize the leguminous plant roots for promoting their growth by certain mechanisms. Present study was carried out to evaluate selenium biofortification in Phaseolus vulgaris under pot study through selenocysteine methyltransferase (SMT) gene expression. Isolation and identification through 16?S rRNA gene sequencing identified MPJ9 as Pantoea dispersa, which was also found to have the capacity to enhance selenium content (3.27-fold) in P. vulgaris as compared to control plants. Fourier-transform infrared (FTIR)?spectroscopy analysis of selenium enriched plant revealed the presence of selenocompound in plant and showing the absorption peaks at 996.93?cm?1 and 2310.80?cm?1 indicating existence of C=Se and Se-H groups, respectively. Thin layer chromatography (TLC)?analysis of selenium enriched plant for presence of S?MT activity was found with Rf value 0.58. Quantitative real-time polymerase chain reaction results of test plant revealed the relative SMT expression in different parts of selenium enriched plants to be in the range of 1.24-fold to 2.80-fold. The maximum SMT expression level was observed 2.80-fold in leaves of selenium enriched plant. Results indicating that the leaves of selenium enriched plant had more capacity to accumulate selenium as selenocompound. Overall growth of plants through inoculation was significantly greater than the control plant, indicating the positive effect of selenorhizobacteria for sustainable crop improvement.
机译：摘要植物生长促进selenorhizobacteria有multi-capacity在豆科植物根促进经济增长由特定机制。进行了评估硒吗生物强化菜豆在锅里研究通过硒代半胱氨酸甲基转移酶(SMT)基因的表达。识别通过16 ?确认MPJ9 Pantoea dispersa,还发现有能力增强在p .寻常的硒含量(3.27倍)控制植物相比。红外(FTIR) ?丰富植物的存在selenocompound在植物和显示吸收峰在996.93厘米?表明C = Se和Se-H团体的存在,分别。(TLC) ?存在的年代吗?0.58. 反应的结果揭示了测试工厂相对SMT表达式的不同部分硒富集植物的范围1.24倍到2.80倍。表达水平观察叶子的2.80倍硒的丰富植物。硒富集植物的叶子更多的积累硒的能力selenocompound。接种显著大于控制植物,指示的积极效应selenorhizobacteria可持续作物改进。

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