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首页> 外文期刊>Shock: Molecular, cellular, and systemic pathobiological aspects and therapeutic approaches = The official journal of the Shock Society, the European Shock Society, the Brazilian Shock Society, the International Federation of Shock Societies >Histamine induced homologous and heterologous regulation of histamine receptor subtype mRNA expression in cultured endothelial cells.
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Histamine induced homologous and heterologous regulation of histamine receptor subtype mRNA expression in cultured endothelial cells.

机译：组胺诱导同源和异源监管组胺受体亚型信使rna在培养的内皮细胞表达。

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摘要

Histamine has been proposed to play an important role in early inflammatory responses, based on studies documenting the effects of histamine on vasodilation and permeability of microvascular endothelium, as well as histamine-stimulated neutrophil adhesion to endothelial cells. The complex and heterogeneous biological mechanisms of histamine-mediated responses are not yet completely characterized. One of the factors defining inflammatory responses stimulated by histamine might be the distribution and density of receptor subtypes on cell membranes. The aim of this study was to assess whether the regulation of histamine receptor mRNA levels represents a control point in histamine-stimulated processes. Histamine-induced regulation of histamine receptor subtype expression was studied in vitro by semiquantitative RT-PCR analysis of total RNA extracted from stimulated and unstimulated endothelial cells. The time dependency and extent of histamine induced down-regulation varied between the H1 and H2 receptor message. The rapid decrease of H2 receptor mRNA lasted for 24 h. On the other hand, the less-pronounced reduction of H1 mRNA was transient and returned to control values after 12 h of histamine stimulation. The H2 receptor message was mainly down-regulated by activation of the H1 receptor protein. Down-regulation of the H1 receptor message seemed to be a net result of H1 and H2 receptor activation. This is the first report demonstrating a complex pattern of homologous and heterologous regulation of histamine receptor expression. These findings reveal new insight into the potential diversity of the mechanism involved in processing information via stimulation of multiple G-protein-linked pathways.
机译：提出了组胺发挥重要在早期炎症反应作用,基于研究记录组胺的影响血管舒张和微血管的渗透性内皮细胞以及histamine-stimulated中性粒细胞粘附内皮细胞。复杂和异构生物机制histamine-mediated反应都没有完全的特征。定义炎症反应刺激组胺可能分布和密度细胞膜的受体亚型。本研究旨在评估是否组胺受体信使rna的监管水平代表一个控制点histamine-stimulated流程。监管组胺受体亚型体外表达进行了研究半定量rt - pcr分析,总RNA从刺激中提取,如果内皮细胞。组胺诱导的下调多样H1和H2受体之间的消息。减少H2受体信使rna持续了24小时。另一方面,较不显著减少H1信使rna是瞬态和恢复控制值12 h组胺刺激后。H2受体主要是下调的消息激活的H1受体蛋白质。下调的消息似乎H1受体的最终结果H1和H2受体激活。展示一个复杂的同源和模式不同的组胺受体的调节表达式。潜在机制的多样性通过参与处理信息刺激多个G-protein-linked通路。

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