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首页> 外文期刊>Journal of cellular physiology. >The impact of cryopreservation in signature markers and immunomodulatory profile of tendon and ligament derived cells
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The impact of cryopreservation in signature markers and immunomodulatory profile of tendon and ligament derived cells

机译：低温贮藏的影响在签名标记和免疫调节的肌腱和韧带派生细胞

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摘要

Tendon and ligament (T/L) engineering strategies towards clinical practice have been challenged by a paucity of understanding in the identification and still poorly described characterization of cellular niches. Prospecting how resident cell populations behave in vitro, and how cryopreservation may influence T/L-promoting factors, can provide insights into T/L-cellular profiles for novel regenerative solutions. Therefore, we studied human T/L-derived cells isolated from patellar tendons and cruciate ligaments as suitable cellular models to anticipate tendon and ligament niches responses for advanced strategies with predictive tenogenic and liga-mentogenic value. Our results show that the crude populations isolated from tendon and ligament tissues hold a stem cell subset and share a similar behavior in terms of tenogenic/ligamentogenic commitment. Both T/L-derived cells successfully undergo cryopreservation/thawing maintaining the tenogenic/ligamentogenic profiles. The major differences between cryopreserved and fresh populations were observed at the gene expression of MKX, SCX, and TNMD as well as at the protein levels of collagen type I and III, in which cells from tendon origin (hTDCs) evidence increased values in comparison to the ones from ligament (hLDCs, p < 0.05). In addition, low-temperature storage was shown to potentiate an immunomodulatory profile of cells, especially in hTDCs leading to an increase in the gene expression of the anti-inflammatory factors IL-4 and IL-10 (p < 0.05), as well as in the protein secretion of IL-10 (p < 0.01) and IL-4 (p < 0.001). Overall, the outcomes highlight the relevance of the cryopreserved T/L-derived cells and their promising immunomodulatory cues as in vitro models for investigating cell-mediated mechanisms driving tissue healing and regeneration.
机译：肌腱和韧带(T / L)工程策略对临床实践的挑战在识别缺乏了解还差特性的描述细胞的利基市场。人口的体外行为,以及如何低温贮藏可能影响T / L-promoting因素,为T / L-cellular可以提供见解新型再生解决方案的概要文件。因此,我们研究人类T / L-derived细胞孤立的髌腱和交叉韧带是合适的细胞模型预计肌腱和韧带利基市场反应与预测tenogenic先进策略和liga-mentogenic价值。原油的数量从肌腱和孤立韧带组织干细胞子集和共享一个相似的行为tenogenic / ligamentogenic承诺。T / L-derived细胞成功地进行低温贮藏/解冻维护tenogenic / ligamentogenic概要文件。低温贮藏和新鲜的之间的区别人群被观察到的基因表达类似MKX、SCX TNMD以及蛋白质胶原蛋白I型和III,细胞从肌腱来源(maui)增加了证据值相比的韧带(hLDCs, p < 0.05)。存储显示统治者一个免疫调节的细胞,尤其是在maui导致基因的增加抗炎因子il - 4的表达和il - 10 (p < 0.05),以及蛋白质分泌的il - 10 (p < 0.01)和il - 4 (p <0.001)。低温贮藏T / L-derived细胞的重要性和他们有前途的免疫调节的线索研究细胞介导的体外模型机制组织愈合和开车再生。

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