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首页> 外文期刊>Journal of cellular physiology. >Nampt affects mitochondria l function in aged oocytes by mediating the downstream effector FoxO3a
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Nampt affects mitochondria l function in aged oocytes by mediating the downstream effector FoxO3a

机译：l Nampt影响线粒体功能卵母细胞通过调节下游效应器FoxO3a

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Maternal aging can impair the quality and decrease the developmental competence of ovulated oocytes. In this study, compromised germinal vesicle breakdown (GVBD) was found in aged mice oocytes. Furthermore, we observed increased reactive oxygen species (ROS) and mitochondrial Ca~(2+) levels, along with reduced mi-tochondrial temperature in aged oocytes. Maternal aging also changed the croto-nylation level in oocytes. Forkhead box O3 (FoxO3a), a member of the forkhead protein family involved in the regulation of cell survival and life span reached a peak level in the metaphase II stage. Compared with a younger group, FoxO3a expression increased in aged oocytes. Intracellular localization of FoxO3a changed from the cytoplasm to chromatin in response to aging. The expression of the upstream regulator nicotinamide-phosphoribosyltransferase (Nampt) peaked in the GVBD stage. Moreover, Nampt expression was increased in aged oocytes, and more intense staining of Nampt was found in aged mice ovary. To further study the role of Nampt in mitochondrial function, specific agonist P7C3 and inhibitor FK866 were applied to aged oocytes, and FK866 significantly decreased adenosine triphosphate and mitochondrial membrane potential. In conclusion, mitochondrial dysfunction in aged oocytes was associated with elevated FoxO3a, and suppression of Nampt could further impair mitochondrial function.
机译：母亲的衰老会削弱质量和减少排卵卵母细胞的发育能力。在这项研究中,破坏胚泡分解(GVBD)在老年小鼠卵母细胞被发现。此外,我们观察到活性增加氧物种(ROS)和线粒体Ca ~ (2 +)mi-tochondrial水平以及减少温度老化卵母细胞。改变了卵母细胞croto-nylation水平。Forkhead盒O3 (FoxO3a)的一员forkhead蛋白质家族参与调节细胞生存和寿命第二峰值水平的中期阶段。随着年轻组,FoxO3a表达增加在卵母细胞。FoxO3a改变从细胞质到染色质应对老龄化。监管机构nicotinamide-phosphoribosyltransferase(Nampt)在GVBD阶段达到顶峰。在年龄在卵母细胞表达增加,更强烈的染色Nampt在岁被发现小鼠卵巢。线粒体功能,特定的受体激动剂P7C3和抑制剂FK866岁被应用于卵母细胞FK866显著降低腺苷三磷酸和线粒体膜的潜力。在年龄在卵母细胞功能障碍有关FoxO3a升高,抑制Nampt可以进一步损害线粒体功能。

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