...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of cellular physiology. >Hsa_circ_001988 attenuates GC progression in vitro and in vivo via sponging miR-197-3p
【24h】

Hsa_circ_001988 attenuates GC progression in vitro and in vivo via sponging miR-197-3p

机译：Hsa_circ_001988变弱GC进展体外并通过骗取体内mir - 197 - 3 - p

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Hsa_circ_001988 has been identified as a tumor suppressor gene in several carcinomas. However, its expression pattern and role in gastric cancer (GC) have still remained elusive. This study aimed to explore the functions of hsa_circ_001988 in GC. Quantitative reverse transcription polymerase chain reaction assay was performed to assess the expressions of hsa_circ_001988, miR-197-3p, FBXW7, CCDC6, and U2AF65 in GC tissues. The correlation analysis was undertaken to find out the relationship between hsa_circ_001988 expression and clin-icopathological factors. A series of cellular experiments were carried out to describe the effects of hsa_circ_001988 on GC in vivo and in vitro. Besides, RNA immunoprecipitation (RIP) assay was performed to verify the relationship among EIF4A3, U2AF65, and hsa_circ_001988. We first found that the expression of hsa_circ_001988 was decreased in 341 GC patients that was related to World Health Organization histological types, Lauren types, and tumor invasion depth (p < .05). Silencing of hsa_circ_001988 facilitated proliferation, colony formation, migration, and invasion of GC cells, while overexpression of hsa_circ_001988 reversed the effect on GC progression in vitro. Additionally, the results of subcutaneous xenotransplanted tumor model demonstrated that overexpressing hsa_circ_001988 significantly suppressed the subcutaneous tumor growth in vivo. Mechanistically, hsa_circ_001988 attenuated the miR-197-3p expression possibly due to its molecular sponge effect, and then, positively promoted FBXW7 expression. Afterwards, FBXW7 regulated the expressions of yes-associated protein 1, cyclinDl, CCDC6, and EMT-related proteins. Notably, RIP assay showed the enrichment relationship among EIF4A3, U2AF65, and hsa_circ_001988. Additionally, EIF4A3 or U2AF65 promoted cyclization of hsa_circ_001988 in GC. Hsa_circ_001988 inhibits the proliferation and metastasis of GC via modulating EIF4A3/U2AF65-mediated hsa_circ_001988/miR-197-3p/FBXW7 axis.
机译：Hsa_circ_001988已被确定为一个肿瘤在几个癌抑制基因。它的表达模式和在胃癌中的作用(GC)仍然难以捉摸。旨在探索hsa_circ_001988的功能在GC。聚合酶链反应检测了评估hsa_circ_001988的表达式,mir - 197 - 3 - p FBXW7 CCDC6, U2AF65 GC组织。找出之间的关系hsa_circ_001988表达和clin-icopathological因素。细胞实验进行了描述hsa_circ_001988 GC体内和的影响体外。试验进行验证的关系在EIF4A3 U2AF65, hsa_circ_001988。第一个发现的表达hsa_circ_001988 341年减少GC的病人这是世界卫生组织有关组织学类型,劳伦类型和肿瘤侵入深度(p < . 05)。hsa_circ_001988促进增殖,殖民地GC细胞形成、迁移和入侵,而过度hsa_circ_001988逆转体外对GC进展的影响。此外,皮下的结果异种移植肿瘤模型证明了overexpressing hsa_circ_001988显著抑制体内皮下肿瘤生长。从力学上看,hsa_circ_001988减毒mir - 197 - 3 - p可能由于其表达式积极分子海绵效应,然后提升FBXW7表达式。监管yes-associated的表达式蛋白质1 cyclinDl CCDC6, EMT-related蛋白质。浓缩EIF4A3之间的关系、U2AF65和hsa_circ_001988。促进了hsa_circ_001988环合的GC。Hsa_circ_001988增殖和抑制通过调制转移的GCEIF4A3 / U2AF65-mediated

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号