Epigallocatechin-3-gallate activates the AMP-activated protein kinase signaling pathway to reduce lipid accumulation in canine hepatocytes

Hongyan Ding; Yu Li; Wei LiHuanqing TaoLeihong LiuCai ZhangTao KongShibin FengJinchun LiXichun WangJinjie Wu

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Epigallocatechin-3-gallate activates the AMP-activated protein kinase signaling pathway to reduce lipid accumulation in canine hepatocytes

机译：Epigallocatechin-3-gallate激活活化蛋白激酶信号通路减少犬肝细胞的脂质积累

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Epigallocatechin-3-gallate (EGCG) plays a crucial role in hepatic lipid metabolism. However, the underlying regulatory mechanism of hepatic lipid metabolism by EGCG in canine is unclear. Primary canine hepatocytes were treated with EGCG (0.01, 0.1, or 1 muM) and BML-275 (an AMP-activated protein kinase [AMPK] inhibitor) to study the effects of EGCG on the gene and protein expressions associated with AMPK signaling pathway. Data showed that treatment with EGCG had greater activation of AMPK, as well as greater expression levels and transcriptional activity of peroxisome proliferator activated receptor-alpha (PPARalpha) along with upregulated messenger RNA (mRNA) abundance and protein abundance of PPARalpha-target genes. EGCG decreased the expression levels and transcriptional activity of sterol regulatory element-binding protein 1c (SREBP-1c) along with downregulated mRNA abundance and protein abundance of SREBP-lc target genes. Of particular interest, exogenous BML-275 could reduce or eliminate the effects of EGCG on lipid metabolism in canine hepatocytes. Furthermore, the content of triglyceride was significantly decreased in the EGCG-treated groups. These results suggest that EGCG might be a potential agent in preventing high-fat diet-induced lipid accumulation in small animals.

机译：Epigallocatechin-3-gallate (EGCG)扮演了一个至关重要的在肝脏脂质代谢中的作用。肝脂质潜在的监管机制新陈代谢EGCG的狗还不清楚。犬肝细胞治疗与EGCG (0.01,0.1,或1妈妈)和bml - 275(一种活化蛋白激酶(AMPK)抑制剂)研究EGCG对基因和蛋白质的影响表达式与AMPK信号有关途径。更大的激活AMPK,以及更多表达水平和转录活性过氧物酶体扩散者激活receptor-alpha随着调节信使RNA (PPARalpha)(mRNA)丰富和蛋白质丰富的PPARalpha-target基因。表达水平和转录活性固醇调节元件结合蛋白1 c(SREBP-1c) mRNA表达下调丰富和蛋白质丰富的SREBP-lc目标基因。bml - 275可以减少或消除的影响EGCG在犬肝细胞脂质代谢。此外,甘油三酸酯的含量EGCG-treated显著下降组。一个潜在的代理在预防脂肪食源性脂质积累的小动物。

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来源
《Journal of cellular physiology.》 |2021年第1期|405-416|共12页
作者
Hongyan Ding; Yu Li; Wei LiHuanqing TaoLeihong LiuCai ZhangTao KongShibin FengJinchun LiXichun WangJinjie Wu;
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College of Animal Science and Technology, Anhui Agricultural University, Hefei, China;

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正文语种英语
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Hepatocytes; Canis familiaris; AMP-Activated Protein KinasesLipid accumulationLipid MetabolismAMPK signalingepigallocatechin gallatePeroxisome Proliferators;

机译：肝细胞;犬属后裔;活化蛋白KinasesLipid accumulationLipid MetabolismAMPKsignalingepigallocatechin gallatePeroxisomeProliferators;

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Epigallocatechin-3-gallate activates the AMP-activated protein kinase signaling pathway to reduce lipid accumulation in canine hepatocytes

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