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首页> 外文期刊>Journal of cellular physiology. >Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF
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Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF

机译：调制的间充质干细胞具备干细胞IL-17脱落落叶及恒牙和bFGF

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Mesenchymal stem cells (MSCs) have been identified within dental pulp tissues of exfoliated deciduous (SHEDs) and permanent (DPSCs) teeth. Although differences in their proliferative and differentiation properties were revealed, variability in SHEDs and DPSCs responsiveness to growth factors and cytokines have not been studied before. Here, we investigated the influence of interleukin-17 (IL-17) and basic fibroblast growth factor (bFGF) on stemness features of SHEDs and DPSCs by analyzing their proliferation, clonogenicity, cell cycle progression, pluripotency markers expression and differentiation after 7-day treatment. Results indicated that IL-17 and bFGF differently affected SHEDs and DPSCs proliferation and clonogenicity, since bFGF increased proliferative and clonogenic potential of both cell types, while IL-17 similarly affected SHEDs, exerting no effects on adult counterparts DPSCs. In addition, both factors stimulated NANOG, OCT4, and SOX2 pluripotency markers expression in SHEDs and DPSCs showing diverse intracellular expression patterns dependent on MSCs type. As for the differentiation capacity, both factors displayed comparable effects on SHEDs and DPSCs, including stimulatory effect of IL-17 on early osteogenesis in contrast to the strong inhibitory effect showed for bFGF, while having no impact on SHEDs and DPSCs chondrogenesis. Moreover, bFGF combined with IL-17 reduced CD90 and stimulated CD73 expression on both types of MSCs, whereas each factor induced IL-6 expression indicating its' role in IL-17/bFGF-modulated properties of SHEDs and DPSCs. All these data demonstrated that dental pulp MSCs from primary and permanent teeth exert intrinsic features, providing novel evidence on how IL-17 and bFGF affect stem cell properties important for regeneration of dental pulp at different ages.
机译：间充质干细胞(msc)已确定在牙髓组织剥落了落叶(棚)和永久(DPSCs)牙齿。尽管增殖的差异,微分属性了,可变性在棚屋和DPSCs响应能力生长因子和细胞因子所无法企及的学习之前。影响interleukin-17 (IL-17)和基础纤维母细胞生长因子(bFGF)具备干细胞棚屋和DPSCs通过分析他们的特点clonogenicity增殖,细胞周期和进展,多能性标记表达式分化后7天治疗。表明IL-17和bFGF不同棚屋和DPSCs扩散的影响clonogenicity,因为bFGF增加增殖和单独使用潜力的细胞类型,虽然IL-17同样影响了,发挥不对成人同行DPSCs的影响。这两个因素刺激NANOG, OCT4、SOX2在棚屋和多能性标记表达式DPSCs显示不同的细胞内表达模式依赖于msc类型。分化能力,这两个因素都显示出来在棚屋和DPSCs可比影响,包括刺激IL-17对早期骨生成的影响对比强烈的抑制作用显示为bFGF,而没有对物流的影响和DPSCs软骨形成。IL-17减少CD90和CD73刺激表达对两种类型的msc,而每个因子诱导il - 6表达表明其“作用在IL-17 / bFGF-modulated棚屋的属性和DPSCs。从小学和恒牙牙髓msc发挥固有特性,提供小说证据IL-17和bFGF如何影响干细胞对牙齿的再生性能很重要纸浆在不同的年龄。

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