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首页> 外文期刊>Journal of cellular physiology. >Genetic deletion of the Tas2r143/Tas2r135/Tas2r126 cluster reveals that TAS2Rs may not mediate bitter tastant-induced bronchodilation
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Genetic deletion of the Tas2r143/Tas2r135/Tas2r126 cluster reveals that TAS2Rs may not mediate bitter tastant-induced bronchodilation

机译：的基因删除Tas2r143 / Tas2r135 / Tas2r126集群显示TAS2Rs不得调解苦tastant-induced bronchodilation

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Bitter taste receptors (TAS2Rs) and their signaling elements are detected throughout the body, and bitter tastants induce a wide variety of biological responses in tissues and organs outside the mouth. However, the roles of TAS2Rs in these responses remain to be tested and established genetically. Here, we employed the CRISPR/Cas9 gene-editing technique to delete three bitter taste receptors— Tas2r143/Tas2r135/Tas2r126 (i.e., Tas2r triple knockout [TKO]) in mice. The fidelity and effectiveness of the Tas2r deletions were validated genetically at DNA and messenger RNA levels and functionally based on the tasting of TAS2R135 and TAS2R126 agonists. Bitter tastants are known to relax airways completely. However, TAS2R135 or TAS2R126 agonists either failed to induce relaxation of precontracted airways in wild-type mice and Tas2r TKO mice or relaxed them dose-dependently, but to the same extent in both types of mice. These results indicate that TAS2Rs are not required for bitter tastant-induced bronchodilation. The Tas2r TKO mice also provide a valuable model to resolve whether TAS2Rs mediate bitter tastant-induced responses in many other extraoral tissues.
机译：苦味受体(TAS2Rs)和他们的信号中检测到的元素的身体,和痛苦的促味剂诱导多种多样组织和器官的生物反应外口。在这些反应还有待测试建立了基因。CRISPR / Cas9基因编辑技术来删除三个苦味受体-Tas2r143 / Tas2r135 Tas2r126(即Tas2r三重敲除小鼠[TKO])。的有效性Tas2r删除验证基因在DNA和信使RNA水平和功能的品尝TAS2R135 TAS2R126受体激动剂。众所周知,航空公司完全放松。TAS2R135或未能TAS2R126受体激动剂诱导放松的婚约航空公司野生型老鼠和Tas2r TKO老鼠或放松在这两方面都存在剂量依赖的相关性,但在相同程度上类型的老鼠。不需要苦tastant-induced吗bronchodilation。一个有价值的模型来解决TAS2Rs是否调解苦tastant-induced反应在许多其他extraoral组织。

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