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首页> 外文期刊>Journal of cellular physiology. >Effect of mesenchymal stem eel I-de rived exosomes on the induction of mouse tolerogenic dendritic cells
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Effect of mesenchymal stem eel I-de rived exosomes on the induction of mouse tolerogenic dendritic cells

机译：间充质干细胞的影响鳗鱼很多‘扯下液在小鼠耐受性树突状的感应细胞

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Dendritic cells (DCs) orchestrate innate inflammatory responses and adaptive immunity through T-cell activation via direct cell-cell interactions and/or cytokine production. Tolerogenic DCs (tolDCs) help maintain immunological tolerance through the induction of T-cell unresponsiveness or apoptosis, and generation of regulatory T cells. Mesenchymal stromal cells (MSCs) are adult multipotent cells located within the stroma of bone marrow (BM), but they can be isolated from virtually all organs. Extracellular vesicles and exosomes are released from inflammatory cells and act as messengers enabling communication between cells. To investigate the effects of MSC-derived exosomes on the induction of mouse tolDCs, murine adipose-derived MSCs were isolated from C57BL/6 mice and exosomes isolated by ExoQuick-TC kits. BM-derived DCs (BMDCs) were prepared and cocultured with MSCs-derived exosomes (100μg/ml) for 72 hr. Mature BMDCs were derived by adding lipopoly-saccharide (LPS; 0.1μg/ml) at Day 8 for 24 hr. The study groups were divided into (a) immature DC (iDC, Ctrl), (b) iDC + exosome (Exo), (c) iDC + LPS (LPS), and (d) iDC + exosome + LPS (EXO + LPS). Expression of CD11c, CD83, CD86, CD40, and MHCII on DCs was analyzed at Day 9. DC proliferation was assessed by coculture with carboxyfluorescein succinimidyl ester-labeled BALB/C-derived splenocytes p. lnterleukin-6 (IL-6), IL-10, and transforming growth factor-β (TGF-β) release were measured by enzyme-linked immunosorbent assay. MSC-derived exosomes decrease DC surface marker expression in cells treated with LPS, compared with control cells (≤ .05). MSC-derived exosomes decrease IL-6 release but augment IL-10 and TGF-β release (p ≤ .05). Lymphocyte proliferation was decreased (p ≤ .05) in the presence of DCs treated with MSC-derived exosomes. CMSC-derived exosomes suppress the maturation of BMDCs, suggesting that they may be important modulators of DC-induced immune responses.
机译：树突状细胞(dc)编排天生的炎症反应和适应性免疫通过激活t细胞通过直接的信息相互作用和/或细胞因子的生产。耐受性DCs (tolDCs)帮助维护通过诱导免疫耐受t细胞反应迟钝或凋亡代的调节性T细胞。基质细胞(msc)是成人的多功能细胞位于骨髓(BM)的基质,但他们可以从几乎所有的孤立器官。释放炎性细胞和作为信使使细胞之间的沟通。调查MSC-derived的影响液的感应鼠标tolDCs,小鼠脂肪msc与C57BL / 6老鼠和液分离ExoQuick-TC包。BM-derived DCs (BMDCs)和准备与MSCs-derived cocultured液(100μg / ml)72人力资源。lipopoly-saccharide(有限合伙人;24小时。未成熟DC (iDC Ctrl), (b) iDC +外来体(挂式),iDC (c) +有限合伙人(有限合伙人)和(d) iDC +外来体+有限合伙人(挂式+ LPS)。CD40, MHCII分析了DCs在天9。扩散是由coculture评估carboxyfluorescein succinimidyl ester-labeledBALB / C-derived脾细胞lnterleukin-6页(il - 6)、il - 10和转化生长因子-β(TGF -β)释放被酶联测免疫吸附试验。减少直流表达在细胞表面标志处理有限合伙人,而控制细胞(≤. 05)。但增加il - 10和TGF -β版本(p≤. 05)。淋巴细胞增殖减少(p≤0。)在DCs MSC-derived对待液。成熟的BMDCs,这表明他们可能是DC-induced免疫的重要调节器响应。

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