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首页> 外文期刊>Journal of cellular physiology. >Overexpression of CFL1 in gastric cancer and the effects of its silencing by siRNA with a nanoparticle delivery system in the gastric cancer cell line
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Overexpression of CFL1 in gastric cancer and the effects of its silencing by siRNA with a nanoparticle delivery system in the gastric cancer cell line

机译：超表达在胃癌和CFL1小干扰rna与沉默的影响纳米颗粒在胃输送系统癌症细胞系

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摘要

Gastric adenocarcinoma, like other cancers, is a multifactorial genetic disease, and metastasis of cancer cells is one of the main features of this illness. The expression levels of the CFL1 gene have been modulated in this pathway. Using small interfering RNA (siRNA) in the treatment of gastric cancer is considered a hopeful gene therapeutic approach. The present study reported the level of CFL1 genes between tumor and margin and healthy tissue of gastric cancer. Also, the features of a cationic nanoparticle with a polymer coating containing polyacrylic acid and polyethylenei-mine that were used in the delivery of CFL1 siRNA, were shown. Then the cytotoxicity, cellular uptake, and gene silencing efficiency of this nanoparticle were evaluated with CFLlsiRNA. Method: In this study, the CFL1 gene expression was measured in 40 gastric adenocarcinoma, marginal and 15 healthy biopsy samples by a real-time polymerase chain reaction. Physicochemical characteristics, apoptosis, and inhibition of migration of the delivery of CFL1 siRNA by nanoparticle and lipofectamine were investigated in gastric cancer cells. Result: The CFL1 expression was remarkably increased in gastric cancer tissues in comparison with the marginal samples and normal tissues (p < .05) and the biomarker index for CFL1 was obtained as 0.94, then this gene can be probably used as a biomarker for gastric cancer. After treatment of the AGS cell line by CFL1 siRNA, the CFL1 expression level of mRNA and migration in AGS cells were remarkably suppressed after transfection. Furthermore, the amount of apoptosis increased (p<.05). Conclusion: Our results demonstrated that CFL1 downregulation in AGS cells can interdict cell migration. Finally, our outcomes propose that CFL1 can function as an oncogenic gene in gastric cancer and would be considered as a potential purpose of gene therapy for gastric cancer treatment.
机译：胃腺癌,和其他癌症一样,是一个多因子的遗传疾病,和转移癌细胞的主要特征之一疾病。在这个途径调制。核RNA治疗胃癌是一个充满希望的基因治疗的方法。CFL1基因肿瘤和边缘之间的水平胃癌组织和健康组织。阳离子纳米颗粒的特性聚合物涂层含有聚丙烯酸和polyethylenei-mine交付使用CFL1 siRNA的显示。细胞吸收和基因沉默效率这与CFLlsiRNA纳米粒子进行了评估。方法:在这项研究中,CFL1基因表达以40胃腺癌,边际和15名健康活检样本实时聚合酶链反应。物理化学特性、细胞凋亡和迁移的抑制CFL1的交付由纳米颗粒和lipofectamine核研究胃癌细胞。CFL1表达显著增加胃癌组织的比较边际样本和正常组织(p < . 05)生物标志物指数CFL1得到0.94,那么这个基因可能可以用作胃癌的生物标志物。CFL1 siRNA AGS细胞系,CFL1信使rna的表达水平在AGS和迁移细胞后被显著抑制转染。细胞凋亡增加(p < . 05)。结果表明,在差别CFL1对这些AGS细胞可以阻断细胞迁移。我们建议CFL1可以作为一个结果致癌基因在胃癌和认为是一个潜在的基因治疗的目的胃癌治疗。

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