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首页> 外文期刊>Journal of cellular physiology. >Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils
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Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils

机译：细胞因子如若msc的不同来源证实了再生的承诺post-coculture与激活中性粒细胞

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The interaction of mesenchymal stromal cells (MSCs) with paracrine signals and immunological cells, and their responses and regenerative commitment thereafter, is understudied. In the current investigation, we compared MSCs from the umbilical cord blood (UCB), dental pulp (DP), and liposuction material (LS) on their ability to respond to activated neutrophils. Cytokine profiling (interleukin-1α [IL-1α], IL-2, IL-4, IL-6, IL-8, tumor necrosis factor-α [TNF-α], interferon-γ [IFN-γ], transforming growth factor-β [TGF-β]), cellular proliferation and osteogenic differentiation patterns were assessed. The results showed largely comparable cytokine profiles with higher TNF-a and IFN-y levels in LSMSCs owing to their mature cellular phenotype. The viability and proliferation between LS/DP/UCB MSCs were comparable in the coculture group, while direct activation of MSCs with lipopolysaccharide (LPS) showed comparable proliferation with significant cell death in UCB MSCs and slightly higher cell death in the other two types of MSC. Furthermore, when MSCs post-neutrophil exposure were induced for osteogenic differentiation, though all the MSCs devoid of the sources differentiated, we observed rapid and significant turnover of DPMSCs positive of osteogenic markers rather than LS and UCB MSCs. We further observed a significant turnover of IL-la and TGF-β at mRNA and cytokine levels, indicating the commitment of MSCs to differentiate through interacting with immunological cells or bacterial products like neutrophils or LPS, respectively. Taken together, these results suggest that MSCs have more or less similar cytokine responses devoid of their anatomical niche. They readily switch over from the cytokine responsive cell phenotype at the immunological microenviron-ment to differentiate and regenerate tissue in response to cellular signals.
机译：间充质基质细胞之间的交互(msc)和旁分泌信号和免疫学细胞,他们的反应和再生承诺之后,是可以理解的。目前的调查,我们相比msc脐带血(UCB),牙髓(DP)和抽脂材料(LS)能力对激活中性粒细胞。定性(interleukin-1α[IL-1α]IL-2 IL-4,il - 6,引发,肿瘤坏死因子-α(TNF -α),干扰素-γ干扰素-γ,改变经济增长因素——β(TGF -β)),细胞增殖和成骨分化模式评估。细胞因子概况与更高的TNF-a和IFN-y水平LSMSCs由于其成熟的细胞表现型。LS / DP /之间的联合msc是可比的coculture集团,而直接激活msc与脂多糖(LPS)显示可比性扩散与联合的重要细胞死亡msc和更高的细胞死亡两种类型的MSC。post-neutrophil曝光被诱导成骨分化,尽管所有的msc没有来源的分化,我们观察到迅速和显著的周转率DPMSCs积极成骨的标记,而不是LS和联合msc。IL-la TGF -βmRNA和细胞因子的水平,表明msc的承诺区分通过相互作用免疫细胞或细菌等产品中性粒细胞或有限合伙人。这些结果表明,msc有或多或少类似的细胞因子反应缺乏的解剖利基。细胞因子的响应性细胞的表型免疫microenviron-ment区分和再生组织细胞信号。

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